結合PCA與雙光子超光譜開發木材分析技術

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姓名

賴威廷(Wei-Ting Lai) 查詢紙本館藏

畢業系所

光電科學與工程學系

論文名稱

結合PCA與雙光子超光譜開發木材分析技術
(Combining PCA and two-photon hyperspectral to develop wood analysis technology)

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至系統瀏覽論文 (2028-7-24以後開放)

摘要(中)

過去對於木材的分析方式可以是觀察其外觀特徵，例如顏色、紋理和氣味等，進而判定木材的種類與品質。然而，這種分析方式的局限性在於無法精確地鑒定木材的化學成分和結構特性，因此無法滿足現代工業對於木材品質控制和加工需求的要求。現今光譜技術已成為主要的木材分析方法，例如紅外光光譜、拉曼光譜和螢光光譜等，能準確判定木材的化學成分、結構和特性等訊息。現代科技的發展也推動了木材分析的自動化和智能化，有助於提高分析效率和精確度，減少人為因素的影響。
本研究使用雙光子超光譜顯微術來做研究工具，分別對三種現代軟木以及九種現代硬木的雙光子螢光光譜進行分析，結合線性分離、PCA和KNN等技術進行辨認模型的建立，探討雙光子螢光光譜區分軟木與硬木的可能性。為了得知過去的製琴技術，我們分別透過鹼處理以及熱處理過後的現代雲杉樣本對歐洲提琴與中國古琴進行比較，藉由此方法，我們可以深入研究過去的製琴技術，進一步加深對於古琴製作的理解。
木材中的纖維結構是管胞細胞壁的主要成分，這些纖維束在細胞壁內依照不同生長條件產生差異，藉由記錄管胞細胞壁中纖維素排列方向有助於我們理解細胞壁的結構，我們藉由雙光子超光譜顯微術拍攝木材纖維素二倍頻(SHG)，研究木材纖維素的分佈以及排列方向。

摘要(英)

In the past, the way to analyze wood can be to observe its appearance characteristics, such as color, texture and smell, etc., and then determine the type and quality of wood. However, the limitation of this analysis method is that it cannot accurately identify the chemical composition and structural properties of wood, so it cannot meet the requirements of modern industry for wood quality control and processing needs. Nowadays, spectroscopic techniques have become the main wood analysis methods, such as infrared spectroscopy, Raman spectroscopy and fluorescence spectroscopy, etc., which can accurately determine the chemical composition, structure and characteristics of wood. The development of modern technology has also promoted the automation and intelligence of wood analysis, which helps to improve the efficiency and accuracy of analysis and reduce the influence of human factors.
This study uses two-photon hyperspectral microscopy as a research tool, analyzes the two-photon fluorescence spectra of three modern softwoods and nine modern hardwoods, and establishes identification models by combining linear separation, PCA and KNN techniques. Possibility of two-photon fluorescence spectroscopy to differentiate softwoods from hardwoods. In order to learn about past violin-making techniques, we compared European violins with Chinese Guqin through alkali-treated and heat-treated modern spruce samples. By this method, we can deeply study past violin-making techniques and further deepen our understanding of Guqin understanding of making.
The fiber structure in wood is the main component of the tracheid cell wall. These fiber bundles are different in the cell wall according to different growth conditions. By recording the cellulose arrangement direction in the tracheid cell wall, we can understand the structure of the cell wall. Photon hyperspectral microscopy photographs wood cellulose double frequency (SHG) to study the distribution and arrangement direction of wood cellulose.

關鍵字(中)

★ 雙光子
★ 主成分分析
★ 二倍頻
★ 超光譜
★ 木材
★ 提琴與古琴

關鍵字(英)

論文目次

中文摘要 i
英文摘要 ii
誌謝 iii
目錄 iv
圖目錄 vi
表目錄 xiii
第一章緒論 1
1.1 研究動機與目的 1
1.2 文獻回顧 2
1.2.1 木材辨認技術 2
1.2.2 木材纖維束 9
第二章實驗原理及木材結構 11
2.1 木材結構 11
2.2 木質部細胞壁 13
2.3 木材聚合物 14
2.3.1 木質素 14
2.3.2 纖維素 15
2.3.3 半纖維素 16
2.3.4 分子結構與纖維排列方向 16
2.4 雙光子螢光顯微術 18
2.5 二倍頻 20
2.6 線掃描超光譜 21
2.7 訊號處理 23
2.7.1 線性分離 23
2.7.2 主成分分析 26
2.7.3 k-鄰近演算法 28
第三章實驗架構和方法 30
3.1 實驗架構 30
3.1.1 雙光子超光譜顯微術 30
3.1.2 旋轉雷射偏振 32
3.2 訊號校正 34
3.2.1 光譜校正 35
3.2.2 sCMOS校正 35
3.3 樣本配置 36
3.3.1 軟硬木分類實驗 37
3.3.2 古琴實驗組 38
3.4 光譜訊號分析方式 39
第四章實驗結果與討論 41
4.1 雙光子螢光影像 41
4.2 木質素螢光光譜 47
4.3 高斯擬合 51
4.4 線性分離 61
4.5 基底強度PCA 67
4.6 改變雷射偏振激發SHG影像 83
第五章結論 89
參考文獻 91

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指導教授

陳思妤(Szu-Yu Chen)

審核日期

2023-8-16

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