| 姓名 |
陳鴻龍(Hong-Long Chen)
查詢紙本館藏 |
畢業系所 |
光電科學與工程學系 |
| 論文名稱 |
氮化物表面增益拉曼光譜於 肝纖維化判定的應用
(The application of nitride Surface-enhanced Raman spectroscopy(SERS) on the diagnosis of liver fibrosis)
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| 相關論文 | |
| 檔案 |
 [Endnote RIS 格式]
[Bibtex 格式]
 [相關文章]  [文章引用]  [完整記錄]  [館藏目錄]  至系統瀏覽論文 (2025-8-1以後開放)
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| 摘要(中) |
本研究利用表面增強拉曼散射（Surface-Enhanced Raman Scattering, SERS）分析纖維化的肝細胞，希望能以這種光譜技術量化肝細胞纖維化的程度。由於物理拉伸的方式無法進行定量分析，我們在肝細胞樣品中添加不同濃度的化學藥劑(Transforming growth factor beta, TGF-β)，以得到不同程度的纖維化細胞。根據文獻，膠原蛋白的過度沉積是肝纖維化的一個主要特徵，且1315 cm-1為膠原蛋白拉曼共振所造成的特徵峰。在實驗中，我們觀察到TGF-β添加下肝纖維化細胞在相關特徵峰強度上呈現成長趨勢。此外，為了進一步深入了解纖維化的程度，我們增加了放置時間，結果顯示隨著時間的延長，纖維化的程度越明顯。SERS是一種高速、精準、非侵入性的檢測技術，本研究以SERS確認了TGF-β濃度、TGF-β浸泡時間和肝細胞纖維化之間的關聯性，有潛力應用至臨床診斷。 |
| 摘要(英) |
This study employs surface-enhanced Raman scattering (SERS) to analyze the fibrosis of human hepatic cells. The goal is to investigate the impact of chemical additive (Transforming growth factor beta, TGF-β) concentration on the behavior of liver fibrosis cells. Since the physical means (stretching) was not suitable for quantification analysis, we immerse the cells in the solution with varied TGF-β concentrations, aiming to see the degree of cell fibrosis vis the SERS spectra. According to the literatures, excessive deposition of collagen protein is a key characteristic of liver fibrosis, with the Raman characteristic peak at 1315 cm-1. In the experiment, we observed the increased SERS intensity at 1315 cm-1 with the liver fibrosis cells in TGF-β. Furthermore, to gain a deeper understanding of the extent of fibrosis, we extended the incubation time, and saw that the cell fibrosis becomes more pronounced with increased incubation time. These findings provide important insights into the relationship between TGF-β concentration, incubation time, and cellular responses in liver fibrosis.The SERS technique, with the advantages of high throughput, high sensitivity and non-invasion,is of high potential for clinical applications. |
| 關鍵字(中) |
★ 氮化物
★ 拉曼增強
★ 肝纖維化 |
關鍵字(英) |
★ GaN
★ SERS
★ Liver Fibrosis |
| 論文目次 |
論文摘要 II
Abstract III
致謝 IV
目錄 V
圖目錄 VI
第一章、緒論 1
1.1表面增強拉曼散射的發展起源 1
1.2氮化物磊晶片應用於SERS的優勢 3
1.3常見的肝纖維化量測技術 4
1.4研究動機 7
第二章、實驗原理、方法與儀器 8
2.1表面增強拉曼散射原理 8
2.2 SERS晶片樣品的製備 13
2.3實驗儀器 17
2.4肝星狀細胞制備 20
2.5 FFPE樣品製備 21
第三章、結果與討論. 23
3.1 選擇金的厚度 23
3.2 Western blot 西方點墨法的量測結果 25
3.3 SERS的量測結果 27
3.3.1化學性與物理性比較 27
3.3.2濃度對纖維化的影響 32
3.3.3時間對纖維化的影響 35
3.4.肝纖維化FFPE樣品 37
4.結論與未來展望 39
4.1結論 39
4.2未來展望 40
參考資料 41 |
| 參考文獻 |
[1] 林鼎晸 ,“表面增強拉曼散射光譜的發展與應用”工業材料雜誌 ,261 150-155(2008)
[2] 董冠廷,“光調變液晶於金屬光柵上之表面電漿波瞬態穿透行為之研究”, 國立中山大學, (2017)
[3]許偉庭,“貴金屬例子電漿共振免標定生物感測器” 化學 ,69,223-233(2011)
[4] Jain P.K. et al.,“Noble Metal Nanoparticle Pairs: Effect of Medium for Enhanced Nanosensing.” Nano Letters 8, 4347 (2008).
[5] 陳瑤真,“表面增強拉曼散射光譜應用於生物單分子偵測”國立交通大學 ，碩士論文(93).
[6] Phan Le Yen Nhi, “GLUCOSE DETECTION BY NITRIDE-BASED SURFACEENHANCED RAMAN SPECTROSCOPY”,國立中央大學,碩士論文，(2022)
[7] Fan Ching, Chien, “Nanostructured InGaN Quantum Wells as a Surface-Enhanced Raman Scattering Substrate with Expanded Hot Spots.” ACS Appl. Nano Mater. 2614–2620 (2021).
[8] Lombardi, J.R. “The theory of surface-enhanced Raman scattering on semiconductor nanoparticles: toward the optimization of SERS sensors.” Faraday Discuss. 205, 105-120 (2017).
[9] Eric C. et al., “Principles of Surface-Enhanced Raman Spectroscopy.” Elsevier Amsterdam (2009).
[10] Dieringer, J. A. et al., “Surface-enhanced Raman excitation spectroscopy of a single rhodamine 6G molecule.” J. Am. Chem. Soc. 131, 849-854 (2009).
[11]網路資料: 什麼是SEM? SEM如何成像?
取自:https://www.jy-idea.com/tw/html/page/page.php?cid=21&cid2=108
[12]維基百科 有機金屬化學氣相沉積法
[13] 趙芳胤，“ The effect of InGaN quantum structures on surface-enhanced Raman spectroscopy biosensors.”碩士論文，(2022)
[14]維基百科 肝臟星狀細胞
[15] Xiaozhou Li, Noninvasive liver diseases detection based on serum surface enhanced Raman spectroscopy and statistical analysis. Optics Express 14,18361-18372(2015)
[16] Qiwen Wang, “Multivariate analysis of serum surface-enhanced Raman spectroscopy of liver cancer patients”. Journal of Innovative Optical Health Sciences. 5,2250032(2022)
[17] Songtao Xiang, “SERS diagnosis of liver fibrosis in the early stage based on gold nanostar liver targeting tags”. Biomaterials Science. 9,5035(2021)
[18] 網路資料: 什麼是西方墨點法(western blot)? 取自 : https://www.thermofisher.com/tw/zt/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-western-blotting.html
[19] Peter Georgeson, et al. Evaluating the utility of tumour mutational signatures for identifying hereditary colorectal cancer and polyposis syndrome carriers. Gut. 2021Nov;70(11):2138-2149.doi: 10.1136/gutjnl-2019-320462. Epub 2021 Jan 7. |
| 指導教授 |
賴昆佑
簡汎清(Kun-You Lai
Fan-Ching Chien)
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審核日期 |
2023-11-13 |
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