Antioxidant and anti-inflammatory potential of hesperetin metabolites obtained from hesperetin-administered rat serum: An ex vivo approach

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Title:	Antioxidant and anti-inflammatory potential of hesperetin metabolites obtained from hesperetin-administered rat serum: An ex vivo approach
Authors:	陳師慶;Yang, Hsin-Ling;Chen, Ssu-Ching;Senthil Kumar, K. J;Yu, Kang-Ni;Lee Chao, Pei-Dawn;Tsai, Shang-Yuan;Hou, Yu-Chi;Hseu, You-Cheng
Contributors:	生醫理工學院生命科學系
Keywords:	administration & dosage;analysis;Animals;antagonists & inhibitors;anti-inflammatory activity;Anti-Inflammatory Agents;Anti-Inflammatory Agents - administration & dosage;Anti-Inflammatory Agents - analysis;Anti-Inflammatory Agents - metabolism;antioxidant activity;Antioxidants;Antioxidants - administration & dosage;Antioxidants - analysis;Antioxidants - metabolism;Biological and medical sciences;Cell Line;chemistry;Citrus;Citrus - chemistry;cytotoxicity;drug effects;Food industries;Fundamental and applied biological sciences. Psychology;hesperetin;hesperidin;Hesperidin - administration & dosage;Hesperidin - analysis;Hesperidin - metabolism;immunology;ingestion;macrophages;Macrophages - drug effects;Macrophages - immunology;metabolism;metabolites;Mice;mitogen-activated protein kinase;Molecular Nutrition;myocytes;NF-kappa B;NF-kappa B - antagonists & inhibitors;NF-kappa B - immunology;nitric oxide;nitric oxide synthase;Nitric Oxide Synthase Type II;Nitric Oxide Synthase Type II - antagonists & inhibitors;Nitric Oxide Synthase Type II - metabolism;phosphorylation;Plant Extracts;Plant Extracts - administration & dosage;Plant Extracts - analysis;Plant Extracts - metabolism;Rats;Rats, Sprague-Dawley;Serum;Serum - chemistry;Serum - drug effects;smooth muscle;sulfates;superoxide anion
Date:	2012-01-11
Issue Date:	2026-04-23 11:11:36 (UTC+8)
Publisher:	American Chemical Society;Washington, DC: American Chemical Society
Abstract:	摘要： In recent years much attention has been focused on the pharmaceutical relevance of bioflavonoids, especially hesperidin and its aglycon hesperetin in terms of their antioxidant and anti-inflammatory actions. However, the bioactivity of their metabolites, the real molecules in vivo hesperetin glucuronides/sulfates produced after ingestion, has been poorly understood. Thus, the study using an ex vivo approach is aimed to compare the antioxidant and anti-inflammatory activities of hesperidin/hesperetin or hesperetin metabolites derived from hesperetin-administered rat serum. We found that hesperetin metabolites (2.5–20 μM) showed higher antioxidant activity against various oxidative systems, including superoxide anion scavenging, reducing power, and metal chelating effects, than that of hesperidin or hesperetin. The data also showed that pretreatment of hesperetin metabolites (1–10 μM) within the range of physiological concentrations, compared to hesperetin, significantly inhibited nitric oxide (NO) and prostaglandin E2 (PGE2) production, as evidenced by the inhibition of their precursors, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein levels without appreciable cytotoxicity on LPS-activated RAW264.7 macrophages or A7r5 smooth muscle cells. Concomitantly, hesperetin metabolites dose-dependently inhibited LPS-induced intracellular reactive oxygen species (ROS). Furthermore, hesperetin metabolites significantly downregulate LPS-induced nuclear factor-κB (NF-κB) activation followed by the suppression of inhibitor-κB (I-κB) degradation and phosphorylation of c-Jun N-terminal kinase1/2 (JNK1/2) and p38 MAPKs after challenge with LPS. Hesperetin metabolites ex vivo showed potent antioxidant and anti-inflammatory activity in comparison with hesperidin/hesperetin. 其他題名： J. Agric. Food Chem 出版者： Washington, DC: American Chemical Society 出版日期： 2012-01-11 出處： Journal of agricultural and food chemistry, 2012-01, Vol.60 (1), p.522-532 資源來源： American Chemical Society Journals 版權： Copyright © 2011 American Chemical Society 版權： 2015 INIST-CNRS 識別號： ISSN: 0021-8561 識別號： ISSN: 1520-5118 識別號： EISSN: 1520-5118 識別號： DOI: 10.1021/jf2040675 識別號： PMID: 22098419 識別號： CODEN: JAFCAU
Appears in Collections:	[Department of Life Science] journal & Dissertation

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