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| 題名: | Perturbation of cytosolic calcium by 2-aminoethoxydiphenyl borate and caffeine affects zebrafish myofibril alignment |
| 作者: | 陳盛良;Wu, Hsin-Ju;Fong, Tsorng-Harn;Chen, Shen-Liang;Wei, Jen-Cheng;Wang, I-Jong;Wen, Chi-Chung;Chang, Chao-Yuan;Chen, Xing-Guang;Chen, Wei-Yu;Chen, Hui-Min;Horng, Juin-Lin;Wang, Yun-Hsin;Chen, Yau-Hung |
| 貢獻者: | 生醫理工學院生命科學系 |
| 關鍵詞: | Alignment;Animals;Borates;Boron Compounds - toxicity;Caffeine;Caffeine - toxicity;Calcium;Calcium - metabolism;Cytosol - drug effects;Cytosol - metabolism;Danio rerio;Embryo, Nonmammalian - drug effects;Embryo, Nonmammalian - metabolism;Embryo, Nonmammalian - ultrastructure;embryogenesis;Embryos;Exposure;Inhibitors;Microscopy, Electron, Transmission;Muscle Development - drug effects;Myofibrils - drug effects;Myofibrils - ultrastructure;myogenesis;Toxicology;Zebrafish;Zebrafish - embryology |
| 日期: | 2015-01-01 |
| 上傳時間: | 2026-04-23 11:13:36 (UTC+8) |
| 出版者: | England: Blackwell Publishing Ltd |
| 摘要: | 摘要： The objective of the current study was to investigate the effects of Ca2+ levels on myofibril alignment during zebrafish embryogenesis. To investigate how altered cytoplasmic Ca2+ levels affect myofibril alignment, we exposed zebrafish embryos to 2‐aminothoxyldiphenyl borate (2‐APB; an inositol 1,4,5‐trisphosphate receptor inhibitor that reduces cytosolic Ca2+ levels) and caffeine (a ryanodine receptor activator that enhances cytosolic Ca2+ levels). The results demonstrated that the most evident changes in zebrafish embryos treated with 2‐APB were shorter body length, curved trunk and malformed somite boundary. In contrast, such malformed phenotypes were evident neither in untreated controls nor in caffeine‐treated embryos. Subtle morphological changes, including changes in muscle fibers, F‐actin and ultrastructures were easily observed by staining with specific monoclonal antibodies (F59 and α‐laminin), fluorescent probes (phalloidin) and by transmission electron microscopy. Our data suggested that: (1) the exposure to 2‐APB and/or caffeine led to myofibril misalignment; (2) 2‐APB‐treated embryos displayed split and short myofibril phenotypes, whereas muscle fibers from caffeine‐treated embryos were twisted and wavy; and (3) zebrafish embryos co‐exposed to 2‐APB and caffeine resulted in normal myofibril alignment. In conclusion, we proposed that cytosolic Ca2+ is important for myogenesis, particularly for myofibril alignment. Copyright © 2014 John Wiley & Sons, Ltd. The objective of the current study was to investigate the correlation of cytosolic calcium levels and myofibril alignments during zebrafish embryogenesis. We exposed 2‐aminoethoxydiphenyl borate and caffeine to zebrafish embryos to disturb the cytosolic calcium levels. Our data show that (1) both enhanced and reduced of cytosolic calcium led to mis‐alignment of myofibrils; (2) reduction of cytosolic calcium increases myod expression; and (3) electron density is affected by intracellular calcium reduction. In conclusion, we proposed that cytosolic calcium is important for myofibrils alignment.
其他題名： J. Appl. Toxicol
出版者： England: Blackwell Publishing Ltd
出版日期： 2015-03
出處： Journal of applied toxicology, 2015-03, Vol.35 (3), p.287-294
資源來源： Wiley Online Library Journals AutoHoldings
版權： Copyright © 2014 John Wiley & Sons, Ltd.
版權： Copyright © 2015 John Wiley & Sons, Ltd.
識別號： ISSN: 0260-437X
識別號： ISSN: 1099-1263
識別號： EISSN: 1099-1263
識別號： DOI: 10.1002/jat.3057
識別號： PMID: 25186829 |
| 顯示於類別: | [生命科學系] 期刊論文
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