Approach to Deliver Two Antioxidant Enzymes with Mesoporous Silica Nanoparticles into Cells

NCU Institutional Repository > 理學院 > 光電科學與工程學系 > 期刊論文 > Item 987654321/103106

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.lib.ncu.edu.tw/handle/987654321/103106

题名:	Approach to Deliver Two Antioxidant Enzymes with Mesoporous Silica Nanoparticles into Cells
作者:	簡汎清;Lin, Yu-Hsuan;Chen, Yi-Ping;Liu, Tsang-Pai;Chien, Fan-Ching;Chou, Chih-Ming;Chen, Chien-Tsu;Mou, Chung-Yuan
贡献者:	理學院光電科學與工程學系
关键词:	Antioxidants - administration & dosage;Antioxidants - chemistry;cell cycle checkpoints;cell membranes;cytoskeleton;fluorescent dyes;glutathione peroxidase;Glutathione Peroxidase - administration & dosage;Glutathione Peroxidase - chemistry;HeLa Cells;Humans;nanocarriers;nanoparticles;Nanoparticles - administration & dosage;Nanoparticles - chemistry;Peptide Fragments - administration & dosage;Peptide Fragments - chemistry;porous media;Protein Denaturation;reactive oxygen species;Recombinant Fusion Proteins - administration & dosage;Recombinant Fusion Proteins - chemistry;silica;Silicon Dioxide - administration & dosage;Silicon Dioxide - chemistry;superoxide dismutase;Superoxide Dismutase - administration & dosage;Superoxide Dismutase - chemistry;tat Gene Products, Human Immunodeficiency Virus - administration & dosage;tat Gene Products, Human Immunodeficiency Virus - chemistry
日期:	2016-07-20
上传时间:	2026-04-23 11:23:26 (UTC+8)
出版者:	American Chemical Society;United States: American Chemical Society
摘要:	摘要： Reactive oxygen species (ROS) are important factors in many clinical diseases. However, direct delivery of antioxidant enzymes into cells is difficult due to poor cell uptake. A proper design of delivery of enzymes by nanoparticles is very desirable for therapeutic purposes. To overcome the cell barrier problem, a designed mesoporous silica nanoparticle (MSN) system with attached TAT-fusion denatured enzyme for enhancing cell membrane penetration has been developed. Simultaneous delivery of two up–downstream antioxidant enzymes, superoxide dismutase (SOD) and glutathione peroxidase(GPx), reveals synergistic efficiency of ROS scavenging, compared to single antioxidant enzyme delivery. TAT peptide conjugation provided a facile nonendocytosis cell uptake and escape from endosome while moving and aggregating along the cytoskeleton that would allow them to be close to each other at the same time, resulting in the cellular antioxidation cascade reaction. The two-enzyme delivery shows a significant synergistic effect for protecting cells against ROS-induced cell damage and cell cycle arrest. The nanocarrier strategy for enzyme delivery demonstrates that intracellular anti-ROS cascade reactions could be regulated by multifunctional MSNs carrying image fluorophore and relevant antioxidation enzymes. 其他題名： ACS Appl. Mater. Interfaces 出版者： United States: American Chemical Society 出版日期： 2016-07-20 出處： ACS applied materials & interfaces, 2016-07, Vol.8 (28), p.17944-17954 資源來源： American Chemical Society Journals 版權： Copyright © 2016 American Chemical Society 識別號： ISSN: 1944-8244 識別號： ISSN: 1944-8252 識別號： EISSN: 1944-8252 識別號： DOI: 10.1021/acsami.6b05834 識別號： PMID: 27353012
显示于类别:	[光電科學與工程學系] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
index.html		0Kb	HTML	31	检视/开启

在NCUIR中所有的数据项都受到原著作权保护.

社群 sharing

数据加载中.....