Use of biotinylated chitosan for substrate-mediated gene delivery

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题名:	Use of biotinylated chitosan for substrate-mediated gene delivery
作者:	鍾志昂;Hu, Wei-Wen;Syu, Wun-Jheng;Chen, Wen-Yih;Ruaan, Ruoh-Chyu;Cheng, Yu-Che;Chien, Chih-Cheng;Li, Chuan;Chung, Chih-Ang;Tsao, Chia-Wen
贡献者:	工學院能源工程研究所
关键词:	Avidin - metabolism;Biological Transport;Biomaterials;Biotin - metabolism;Biotinylation;Carbohydrates;Chitosan - chemistry;Chitosan - metabolism;Chitosan - toxicity;Cytotoxicity;Deoxyribonucleic acid;DNA;DNA - genetics;DNA - metabolism;Drug Carriers - chemistry;Drug Carriers - metabolism;Drug Carriers - toxicity;HEK293 Cells;Humans;Ligands;Materials Testing;Molecules;Nanoparticles;Nanoparticles - chemistry;Pharmaceuticals;Surface Properties;Transfection - methods;Vitamin B
日期:	2012-08-15
上传时间:	2026-04-23 12:05:28 (UTC+8)
出版者:	American Chemical Society;United States: American Chemical Society
摘要:	摘要： To improve transfection efficiency of nonviral vectors, biotinylated chitosan was applied to complex with DNA in different N/P ratios. The morphologies and the sizes of formed nanoparticles were suitable for cell uptake. The biotinylation decreased the surface charges of nanoparticles and hence reduced the cytotoxicity. The loading capacities of chitosan were slightly decreased with the increase of biotinylation, but most of the DNA molecules were still complexed. Using different avidin-coated surfaces, the interaction between biotinylated nanoparticles to the substrate may be manipulated. The in vitro transfection results demonstrated that biotinylated nanoparticles may be bound to avidin coated surfaces, and the transfection efficiencies were thus increased. Through regulating the N/P ratio, biotinylation levels, and surface avidin, the gene delivery can be optimized. Compared to the nonmodified chitosan, biotinylated nanoparticles on biomaterial surfaces can increase their chances to contact adhered cells. This spatially controlled gene delivery improved the gene transfer efficiency of nonviral vectors and could be broadly applied to different biomaterial scaffolds for tissue engineering applications. 其他題名： Bioconjugate Chem 出版者： United States: American Chemical Society 出版日期： 2012-08-15 出處： Bioconjugate chemistry, 2012-08, Vol.23 (8), p.1587-1599 資源來源： American Chemical Society Journals 版權： Copyright © 2012 American Chemical Society 版權： Copyright American Chemical Society Aug 15, 2012 識別號： ISSN: 1043-1802 識別號： ISSN: 1520-4812 識別號： EISSN: 1520-4812 識別號： DOI: 10.1021/bc300121y 識別號： PMID: 22768969
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