Development of a chip-based multiplexed immunoassay using liposomal nanovesicles and its application in the detection of pathogens causing female lower genital tract infections

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題名:	Development of a chip-based multiplexed immunoassay using liposomal nanovesicles and its application in the detection of pathogens causing female lower genital tract infections
作者:	鄒宗山;Su, Wen-Hsiang;Ho, Tien-Yu;Tsou, Tsung-Shan;Lee, Wen-Ling;Wang, Kuan-Chin;Yu, Yuan-Yi;Chen, Tien-Jui;Tan, Chia-Hsuan;Kuo, Cheng-Deng;Chen, Chien-Sheng;Wang, Peng-Hui
貢獻者:	理學院統計研究所
關鍵詞:	Antibodies, Bacterial;Antibodies, Fungal;Candida albicans - immunology;Candidiasis, Vulvovaginal - diagnosis;cervicovaginitis;Feasibility Studies;Female;Gram-Negative Bacteria - immunology;Gram-Negative Bacterial Infections - diagnosis;Humans;Immunoassay - instrumentation;Immunoassay - methods;Internal Medicine;liposome;Liposomes;Microarray Analysis - instrumentation;Microarray Analysis - methods;multiplexed immunoassay chip;Nanostructures;nanovesicle;Obstetrics and Gynecology;Sensitivity and Specificity;Streptococcal Infections - diagnosis;Streptococcus agalactiae - immunology;Uterine Cervicitis - diagnosis;Uterine Cervicitis - microbiology;Vaginitis - diagnosis;Vaginitis - microbiology
日期:	2013-03-01
上傳時間:	2026-04-23 12:52:43 (UTC+8)
出版者:	China (Republic : 1949- ): Elsevier B.V
摘要:	摘要： Cervicovaginitis is a highly prevalent disease that is a burden on healthcare globally. Immediate and adequate treatment can eradicate the infection and block subsequent complications. The feasibility of achip-based multiplexed immunoassay using liposomal nanovesicles was tested. A multiplexed immunoassay chip containing five antibodies for five pathogens (Chlamydia trachomatis, Escherichia coli, Neisseria gonorrhoeae, Streptococcus agalactiae, and Candida albicans) was established and tested. Four patients with spiking of candidiasis were enrolled. The difference between positive and negative readings was evaluated using the paired Student t test. The detection threshold of Candida in this microarray was 100,000 CFU/mL in a vaginal sample, and the time required for the whole procedure was 3 hours. The testing of the four patients showed 100% for both sensitivity and specificity. This microarray chip was a rapid, easy, inexpensive and sensitive tool for detecting female lower genital tract Candida infection in a one-time vaginal sampling process, although the data on the four other pathogens were still unavailable. A larger population study is encouraged to test the validity of this multiplexed immunoassay chip. 其他題名： Taiwan J Obstet Gynecol 出版者： China (Republic : 1949- ): Elsevier B.V 出版日期： 2013-03-01 出處： Taiwanese journal of obstetrics & gynecology, 2013-03, Vol.52 (1), p.25-32 資源來源： Elsevier ScienceDirect Journals 版權： 2013 版權： Copyright © 2013. Published by Elsevier B.V. 識別號： ISSN: 1028-4559 識別號： ISSN: 1875-6263 識別號： EISSN: 1875-6263 識別號： DOI: 10.1016/j.tjog.2013.01.005 識別號： PMID: 23548214
顯示於類別:	[統計研究所] 期刊論文

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