在果蠅(Drosophila melanogaster)中，sable1是一個位於 X染色體上的突變，但造成該突變的基因尚不明確。為了進行基因定位，該研究利用了FLP-FRT染色體重組技術製作了一系列具有染色體缺失的果蠅品系，並以此進行性狀互補性測試同時，以RNAi knockdown的方式鎖定候選基因並藉由Sanger定序法定序PCR產物，比對sable1突變品系和對照組w1118品系的序列差異，鎖定了Yippee基因為造成sable1突變的最有力基因。為了進一步了解Yippee基因，該研究對於成蟲向上爬行的能力、成蟲的壽命和雌蟲的生殖能力進行了測試。藉由遺傳背景置換將Ypes-1等位基因至三個不同的品系──153.6、Oregon-R和w1118──之中，發現Ypes-1個體在三個不同基因背景之下都出現了體色變深，胸部背板出現三叉戟圖樣的紋路。Ypes-1果蠅在三個基因背景中都出現了壽命減少和雌蟲生殖力下降的情況，也印證了雌蟲生殖力下降源自於Ypes-1雌蟲卵巢大小的減少有趣的是，153.6和Oregon-R基因背景的Ypes-1成蟲出現了向上爬行能力減弱的狀況，但在w1118基因背景的Ypes-1成蟲向上爬行的能力則增強了。概括而言，除了已知Yippee基因與體表色素沉澱的有關的資訊外，該研究進一步提供了Yippee基因在成蟲向上爬行能力、壽命與雌蟲生殖力方面的基因多效性研究結果。;The sable1 is a classic X-linked hypomorphic mutation whose causal locus was originally unspecified. To identify the causal locus， a series of chromosomal deletion strains was generated by FLP-FRT recombination technique for complementation test， and performed RNAi-mediated knockdown of the candidate genes. The further verification results by Sanger sequencing data for gene mapping suggesting that the sable1 mutation resides within the Yippee gene locus. Motivated by a fundamental interest in the poorly understood functions of Yippee and preliminary observations of physiological impairments in our laboratory strains. A series of systematic evaluation was conducted of its impact on adult climbing ability， longevity， and female fertility. By introgressing the Ypes-1 allele into three distinct genetic backgrounds—153.6， Oregon-R， and w1118—Cuticular hyper-melanization and a prominent dark thoracic trident pattern were consistently induced by Ypes-1， regardless of the genetic backgrounds. Furthermore， the mutation caused a profound reduction in longevity and female fertility; the latter associated with stunted egg chamber development and reduced ovary size. Notably， while climbing ability displacement diminished in 153.6 and Oregon-R Ypes-1 mutants， this effect was reversed in the w1118 background， where w1118， Ypes-1 double mutants exhibited significantly stronger climbing ability than w1118 controls. In summary， these findings provide a more comprehensive profile of the pleiotropic effects associated with Ypes-1， establishing that Yippee is critical for locomotion， aging， and oogenesis， in addition to its established role in cuticular pigmentation.
