本研究開發出一種兼具超順磁性與局域表面電漿共振（LSPR）光熱效應的多功能複合奈米粒子（γ-Fe₂O₃@SiO₂@Au），作為光熱驅動核酸擴增之奈米材料平台。在材料製備方面以熱分解法合成超順磁性氧化鐵核心，再採用逆微乳液法於其表面包覆厚度可控之二氧化矽中介層，以提升膠體穩定性並提供表面修飾位點。接著對矽殼層表面進行胺基化修飾，並藉由反式環辛烯（TCO）與四嗪（Tz）的點擊化學反應，將具表面負電性的金奈米種子均勻接枝於磁珠表面，並透過種子成長法建構出金殼層結構。該複合粒子的磁性核心賦予其快速磁分離與富集能力，有利於後續生物樣品之前處理與分析整合。最後本研究建立雷射光熱測試平台，驗證此複合奈米粒子具備穩定恆溫與可重複熱循環之溫度控制功能。本研究所建立之磁性表面電漿奈米複合材料及光熱測試平台，可作為核酸擴增之基礎，未來可望應用於熱循環與恆溫核酸擴增檢測之相關領域。;This study develops a multifunctional composite nanoparticle， γ-Fe₂O₃@SiO₂@Au， which exhibits both superparamagnetic properties and localized surface plasmon resonance (LSPR)-induced photothermal effects， serving as a nanomaterial platform for photothermal-driven nucleic acid amplification. For material synthesis， superparamagnetic iron oxide cores (γ-Fe₂O₃) were first prepared via thermal decomposition. To enhance colloidal stability in polar solvents and provide surface modification sites， a thickness-controllable silica intermediate layer was coated onto the cores using the inverse microemulsion method. Subsequently， the silica surface was aminated， and negatively charged gold seeds were uniformly grafted onto the magnetic beads via a click chemistry reaction between trans-cyclooctene (TCO) and Tetrazine (Tz). A uniform gold shell was then successfully constructed around the nanoparticles using the seed-mediated growth method. Furthermore， the magnetic core enables rapid magnetic separation and enrichment， which facilitates subsequent biological sample pretreatment and analytical integration. Finally， a laser-driven photothermal testing platform was established， verifying that the composite nanoparticles possess excellent temperature control capabilities for both stable isothermal maintenance and repeatable thermal cycling. In conclusion， the magnetic surface plasmon nanocomposite and the photothermal platform established in this study lay a solid foundation for photothermal nucleic acid amplification， offering great potential for future applications in thermal cycling and isothermal nucleic acid detection.
