針對生化分析與癌症生物標記檢測對高靈敏度、多重辨識及訊號編碼的需求，本研究建立一種以銀介觀晶體(Silver Mesocrystals， AgMCs)為基礎之表面增強拉曼散射(Surface-Enhanced Raman Scattering， SERS)編碼奈米探針，並將其應用於癌症相關生物標記之多重檢測。由於銀介觀晶體具有一定有序排列的奈米晶粒所組成，兼具介晶結構所形成的多尺度表面結構與銀材料的局部表面電磁增強特性，可作為SERS編碼探針的基底。 本研究透過形貌調控建立具有不同表面結構特徵的AgMCs，並利用其表面等離子體特性與拉曼報告分子的光譜差異建立編碼探針。經由不同Raman reporter分子的組合，本研究建立包含7種光譜編碼的SERS探針庫，並依據各reporter分子的特徵拉曼峰進行辨識與訊號分析。實驗結果顯示，各編碼探針可產生具有區別性的拉曼光譜訊號，並可進一步應用於多重SERS分析。 在生物標記檢測方面，本研究將編碼SERS奈米探針應用於前列腺癌相關生物標記之檢測，藉由不同reporter分子的特徵訊號進行標記與辨識。結果顯示，該探針系統可用於癌症相關生物標記的SERS分析，並具備多重檢測與光譜編碼的應用可行性。整體而言，本研究建立了以AgMCs為SERS基底之編碼奈米探針平台，並展示其於體外癌症生物標記多重檢測中的應用。;To address the demands for high sensitivity， multiplex recognition， and signal encoding in biochemical analysis and cancer biomarker detection， this study developed a surface-enhanced Raman scattering (SERS) encoded nanoprobe based on silver mesocrystals (AgMCs) for multiplex detection of cancer-related biomarkers. AgMCs consist of ordered assemblies of nanocrystals， combining the multiscale surface features of mesocrystalline structures with the localized electromagnetic enhancement properties of silver， making them suitable as SERS substrates for encoded nanoprobes. In this study， AgMCs with distinct surface structural characteristics were prepared through morphological control. Their surface plasmonic properties and the distinct spectral signatures of Raman reporter molecules were exploited to construct encoded probes. By incorporating different Raman reporter molecules， a library of seven spectrally encoded SERS probes was established. These probes were identified and analyzed based on the characteristic Raman peaks of the respective reporter molecules. The results demonstrated that the encoded probes generated distinct Raman spectral signatures， enabling their application in multiplex SERS analysis. For biomarker detection， the encoded SERS nanoprobes were applied to the detection of prostate cancer-related biomarkers， with target recognition achieved through the characteristic signals of different Raman reporter molecules. The results demonstrated the feasibility of using the probe system for SERS-based analysis of cancer-related biomarkers， with potential for multiplex detection and spectral encoding. Overall， this study established an AgMC-based encoded nanoprobe platform and demonstrated its application in the multiplex detection of cancer-related biomarkers in vitro.
