細胞在體內會受到機械力、電訊號及流體環境等多種物理刺激的調控，這些刺激對於細胞的生長、排列、分化及功能維持具有重要影響。然而，傳統靜態細胞培養系統難以重現體內複雜且動態的微環境，因此如何建立更具生理相關性的細胞培養平台已成為近年來生物醫學工程領域的重要研究課題。 本研究以先前開發之模組化複合式物理刺激生物反應器為基礎，首先針對培養裝置結構、刺激模組及控制系統進行優化，以提升系統穩定性、操作便利性及刺激參數調整彈性。系統整合機械拉伸刺激、電刺激及自動化控制功能，並以樹莓派（Raspberry Pi）作為控制核心，開發具圖形化操作介面之控制系統，可進行刺激參數設定、培養排程管理及自動化執行，以提高實驗的穩定性與重複性。 在完成生物反應器優化後，本研究進一步導入微流體技術與灌流培養概念，將原有生物反應器進行微型化與晶片化設計，建立具循環培養液系統之 Muscle-on-a-Chip 平台。晶片以聚二甲基矽氧烷（Polydimethylsiloxane， PDMS）作為主要結構材料，並結合導電聚吡咯（Polypyrrole， PPy）薄膜作為電刺激介面，使系統同時具備機械拉伸、電刺激及培養液灌流等多重物理刺激功能。相較於傳統生物反應器，所開發之晶片系統有效降低了裝置體積、培養液消耗量及系統重量，同時保留原有多重刺激培養能力，提升系統整合性與未來應用彈性。;Cells are regulated by various physical stimuli in vivo， including mechanical forces， electrical signals， and fluid flow， all of which influence cellular growth， differentiation， and function. However， conventional static culture systems are unable to accurately reproduce the complex physiological microenvironment of living tissues. Therefore， developing advanced cell culture platforms with enhanced physiological relevance has become an important research objective. In this study， a previously developed modular multi-physical stimulation bioreactor was further optimized to improve system stability， operational flexibility， and stimulation parameter control. Mechanical stretching， electrical stimulation， and automated scheduling functions were integrated into a Raspberry Pi-based control system with a graphical user interface， enabling user-friendly operation and improved experimental reproducibility. Based on the optimized bioreactor， microfluidic and perfusion culture concepts were introduced to miniaturize the platform into a Muscle-on-a-Chip system. The chip was fabricated using polydimethylsiloxane (PDMS) and incorporated a conductive polypyrrole (PPy) layer for electrical stimulation. The resulting platform provides mechanical stretching， electrical stimulation， and continuous medium perfusion within a compact and integrated system while reducing device size and culture medium consumption.
