在醫學治療上，應用雷射所產生的光熱效應會使組織因為熱能生成導致組成以及結構改變，而在光學性質的呈現上有所不同。掌握組織的光學性質，除了可以提供相關訊息來調整雷射劑量以外，獲知各種正常組織的光學性質對於造影診斷方面亦有所助益。 以積分球搭配傅立葉轉換紅外線光譜分析儀來測量高散射性肌肉組織的實驗參數，並以Inverse Adding-Doubling方法來計算其吸收係數、散射係數以及非等向因子，最後將得到的結果用來觀察凝結前後豬心肌、骨骼肌與平滑肌，得知肌肉組織在光學性質上的差異。 在比較過三種肌肉組織後，發現組織內蛋白質於波長750 nm處，控制著吸收係數的變化；而光照射在肌肉上，因纖維紋理方向的不同，確實影響光子散射的分布；在凝結後的肌肉組織裡，因蛋白質變性後所造成組織的白濁，會導致穿透率降低，並增加光被散射的情形，順向散射的能力因此變得較弱。整體而言，凝結後組織衰減光的能力增加，而在吸收係數方面，特徵吸收峰明顯的消失。 In medical treatment, photo-thermal effect produced by laser irradiation may change the composition and structure of tissue. The optical properties of tissue may also change accordingly. Knowledge of the optical property change of tissue enables one to adjust the dose of laser. Furthermore, it is helpful in image diagnosis. In this study, we measure the transmittance and reflectance of highly scattering muscular tissues by using an integrating sphere and a Fourier transform infrared spectrometer (FTIR). The absorption coefficient, scattering coefficient and anisotropic factor are obtained by the inverse adding-doubling method. This procedure is applied to heated and unheated porcine myocardium, skeletal muscle, and smooth muscle. Effects of coagulation on the optical properties of the muscles are investigated. The results show that an absorption peak at 750 nm can be found for all three muscles. This absorption peak is due to protein. However, this peak disappears for coagulated samples after heating. The fiber orientation does affect the scattering coefficient and the anisotropic factor. Denatured protein in coagulated muscular tissues causes the tissue turn white and turbid. This reduces transmittance but increases scattering. The scattering becomes less forward (anisotropic factor decreases).
