Dynamic fluorescence imaging analysis to investigate the cholesterol recruitment in lipid monolayer during the interaction between beta-amyloid (1-40) and lipid monolayers

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题名:	Dynamic fluorescence imaging analysis to investigate the cholesterol recruitment in lipid monolayer during the interaction between beta-amyloid (1-40) and lipid monolayers
作者:	Lin,MS;Chen,XB;Wang,SSS;Chang,Y;Chen,WY
贡献者:	化學工程與材料工程學系
关键词:	SINGLE-PARTICLE TRACKING;ALZHEIMERS-DISEASE;FIBRIL FORMATION;CORTICAL-NEURONS;ENDOGENOUS SEED;SURFACE TENSION;PROTEIN;MEMBRANES;PEPTIDES;NEUROTOXICITY
日期:	2009
上传时间:	2010-06-29 17:26:42 (UTC+8)
出版者:	中央大學
摘要:	Extracellular beta-amyloid (A beta) deposit is considered as one of the primary factors in inducing Alzheimer's disease (AD). However. the mechanism of A beta deposition on the cell membrane and the induced cytotoxicity are still unclear. On the basis of the previous reports and results, we propose the "Recruiting Hypothesis" on the interaction between the plasma membrane and A beta. Recently, many studies focused on cholesterol, which is considered as an important factor for AD. The most challenging issue in studying the cholesterol is non-ideal mixing behavior and non-dynamic analysis. In the present study, we investigated the cholesterol recruitment in the lipid monolayer during the interaction between beta-amyloid peptides A beta (1-40) and lipid monolayers by dynamic fluorescent imaging analysis. Results from lipid monolayer trough studies showed that the rate of A beta adsorption onto lipid monolayer is mainly due to the electrostatic effect which is sensitive to the lipid monolayer composition. From the fluorescence imaging analysis, the interaction of A beta with lipid monolayer containing negative charge lipid and cholesterol brings out the recruiting behavior of the cholesterol and reduces the fluidity of lipid. The present study not only demonstrates the technical application for monitoring the dynamic molecular behaviors at the interface but also reveals the roles to distinguish lipid molecules on the A beta-membrane interaction. (C) 2009 Elsevier B.V. All rights reserved.
關聯:	COLLOIDS AND SURFACES B-BIOINTERFACES
显示于类别:	[化學工程與材料工程研究所] 期刊論文

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