In vitro study of enzymatic degradation of biological tissues fixed by glutaraldehyde or epoxy compound

NCUIR > Engineering College > National Central University Department of Chemical & Materials Engineering > journal & Dissertation > Item 987654321/26557

Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/26557

Title:	In vitro study of enzymatic degradation of biological tissues fixed by glutaraldehyde or epoxy compound
Authors:	Sung,HW;Chen,WY;Tsai,CC;Hsu,HL
Contributors:	化學工程與材料工程學系
Keywords:	DERMAL SHEEP COLLAGEN;MECHANICAL-PROPERTIES;VALVED CONDUIT;HEART-VALVE;BIOMATERIAL;CALCIFICATION;PREVENTION;INVITRO;INVIVO
Date:	1997
Issue Date:	2010-06-29 17:31:01 (UTC+8)
Publisher:	中央大學
Abstract:	The study, using bacterial collagenase, was to investigate the changes in characteristics of a collagen-rich tissue, porcine pericardium, fixed by glutaraldehyde or epoxy compound (ethylene glycol diglycidyl ether) during the course of degradation. Fresh porcine pericardium was used as a control. During degradation, the heat released by the reaction of collagenase with a test sample was monitored by a highly sensitive microcalorimeter. Also, the degree of degradation of each test sample was determined by measuring its increment in free amino group content and changes in denaturation temperature and tensile strength. Microcalorimetric analysis of collagenase degradation of fresh, epoxy-fixed, and glutaraldehyde-fixed tissues revealed that the heat released during degradation correlates well with the degree of tissue degraded. The cleaving of peptide bonds in biological tissue by collagenase degradation may increase its free amino group content and reduce its denaturation temperature and tensile strength. It was noted that the fresh tissue cannot resist bacterial collagenase degradation, while the glutaraldehyde fixed tissue had a relatively better resistance to degradation than its epoxy-fixed counterpart.
Relation:	JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
Appears in Collections:	[National Central University Department of Chemical & Materials Engineering] journal & Dissertation

Files in This Item:

File	Description	Size	Format
index.html		0Kb	HTML	442	View/Open

社群 sharing

Loading...