XTT-colorimetric assay as a marker of viability in cryoprocessed cardiac valve

NCU Institutional Repository > 工學院 > 化學工程與材料工程研究所 > 期刊論文 > Item 987654321/26583

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.lib.ncu.edu.tw/handle/987654321/26583

题名:	XTT-colorimetric assay as a marker of viability in cryoprocessed cardiac valve
作者:	Lu,JH;Chiu,YT;Sung,HW;Hwang,B;Chong,CK;Chen,SP;Mao,SJ;Yang,OZ;Chang,Y
贡献者:	化學工程與材料工程學系
关键词:	AORTIC HEART-VALVES;CELLULAR CHARACTERIZATION;ANTIBIOTIC PRESERVATION;WARM ISCHEMIA;TISSUE;CRYOPRESERVATION;MAINTENANCE;SURVIVAL;MATRIX
日期:	1997
上传时间:	2010-06-29 17:31:39 (UTC+8)
出版者:	中央大學
摘要:	This study sought to evaluate the use of tetrazolium salt XTT reduction as an indicator of valvular viability in a cryoprocessed porcine cardiac homograft model, The XTT tetrazolium assays was based on the metabolic reduction of Sodium 3'-[1-(phenylamino-carbonyl)-3,4-Tetrazolium]-bis(4- methoxy-6-nitro) benzene sulfonic acid hydrate, The relationship between XTT reduction and: (1) leaflet tissue with various weight (n = 24); (2) morphometric evaluation (n = 30); (3) cadaveric ischemic intervals (n = 30); (4) freeze-thawing (n = 30) has been studied. The measurement of XTT reduction were significantly correlated with the weight of cardiac leaflets, in the range of 30 to 180 mg (y = 0.015x-0.063; r = 0.99). Compared to morphometry of valvular damage, the reduction of mitochondrial enyzmatic activity in cardiac leaflets was correlated with matrix cells without irreversible damage (r = 0.89, P < 0.005), The depletion of XTT reduction occurred dependent of ischemic time intervals, In general, freeze-thawing reduced more than 20% activity of mitochondrial dehydrogenase. We concluded that XTT tetrazolium assay is highly sensitive to determine valvular injury, The study demonstrated its potential for testing of cryopreserved cardiac valve. (C) 1997 Academic Press Limited.
關聯:	JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
显示于类别:	[化學工程與材料工程研究所] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
index.html		0Kb	HTML	585	检视/开启

在NCUIR中所有的数据项都受到原著作权保护.

社群 sharing

数据加载中.....