生物科技的發展，不但能增進人類的社會福祉，同時更具有龐大的經濟發展潛力，在新科技與商機不斷突破的推波助瀾下，將成為新世紀全球大力投入發展的新興高科技產業。國內現有的液態氮超低溫(-196℃)儲存設備，例如Taylor-Wharton系統之傳統開蓋式的液態氮儲存槽以及機械式儲存槽Cryo-Cell系統(Cryo-Cell International, Inc. USA)、BioArchive系統(Thermogenesis Corp. USA)…等。 本篇論文主要的研究是開發新式液態氮超低溫(-196℃)儲存設備，藉由回顧其它家的產品，保留其使用上與保存上的優點並改善其缺點，設計出一個更適合存放生物試片的超低溫(-196℃)儲存設備。 研究過程中包含軟體與硬體的設計，硬體方面：使用3D繪圖軟體將新式液態氮超低溫(-196℃)儲存設備的模型繪製出來，再使用有限元素分析法，分析其結構強度，瞭解應力集中處是否會造成材料的破壞，最後製作出實體設備並做磨耗測試；軟體方面：撰寫可以和儲存槽相互搭配的人機介面程式。 研究的成果：有限元素分析結果顯示此設計是可行的。再者儲存槽在不使用電力的情形下，利用最簡單的機構設計，使試片夾做單一的存取方式，此種方式可以避免影響到其它試片的生物活性，最後使硬體設備與操作軟體互相搭配達到人機介面整合。 The progress of biotechnology not merely advents the human welfare, but reveals great economic potential. With the development of technology and the attraction of business profits, the biotechnology is destined to become a nouveau high-tech industry where the whole world will pour into enormous efforts. This study aims to develop a container with liquid nitrogen at -196°C as a more suitable storage for slice of organism, by extracting advantage and eliminating limitation from currently-adopted containers. At present, there are the traditional manual lid-opening container by Taylor-Wharton, the mechanical container by Cryo-Cell (Cryo-Cell International Inc., USA), the one by BioArchive (Thermogenesis Corp., USA) and others in Taiwan. The study procedure consists of two parts: container’s manufacture and software development. The former is achieved by the following steps: (1) draw the container model with 3D software; (2) calculate the structural strength with the finite-element analysis; (3) examine whether the stress concentration exceeds the material strength of the container; (4) manufacture the container and conduct the wear tests. The latter is to develop window-style software to control and monitor the operation and function of the container. The results of the current study demonstrate the practicability of the new-designed container. Using this container, the operator could easily and conveniently put into and take off a single cartridge without any power supply, thus preventing the other cartridges in the same container from a decrease in the biological activity. Moreover, the development of the user-friendly software can facilitate and enhance the practicability and convenience of the hardware and software.
