本研究主旨為設計一套壓力式生物反應器，為了得到穩定且正確的壓力值，我們架構了回授控制系統，使得生物反應器可施加定壓或循環壓力。測試結果顯示，壓力值最大可達到5 MPa，頻率可達1 Hz。並利用無菌採樣袋成功節省實驗中培養液使用量，經由實驗證明無菌採樣袋內環境適合細胞培養。 我們使用此生物反應器給予大鼠骨髓幹細胞 (RM1) 定常壓力 (0.4、0.55、0.7、1 MPa)，其中1 Ma實驗，施以二組不同的加壓斜率到達定值1 MPa，測試加壓斜率對大鼠髓幹細胞生長速率的影響。實驗結果證明初始加壓斜率過大會抑制大鼠骨髓幹細胞生長。往後可利用此回授控制壓力式生物反應器給予不同壓力式形刺激細胞，觀察壓力對細胞基因表現的影響。 The study design a pressure bioreactor, in order to steady and correct pressure value, we frame automatic control hydrostatic system that can provide constant and time-periodic pressure for in vitro cell cultures. The systems’ performance was stable at a constant hydrostatic pressure up to 5 MPa and cyclic hydrosatatic pressure up to 1 Hz. Cells were putting in the whirl-Pak Sample Bags, which could largely reduce the amount of culture media and prevent contamination. We tested the rat bone marrow stem cells (RM1) by applying 0.4, 0.55, 0.7 and 1 MPa of static pressure at the bioreactor. We study how the pressure slope in time may influence the cell growth by, we changed the pressure slope for the 1 MPa case. The results show it is likely to be the pressure slope rather than the final static pressure value that inhibited the RM1 growth. The cells growth apparently slowed down when the pressure slopes were large than 700 KPa/s. The bioreactor was proved to be available in cells culture and it will be applied to test cells for gene expressions in the future.
