本計畫擬利用功能性基因體學的研究方式， 由T-DNA 插入之水稻突變種原庫與已公佈與熱耐性相關之突變株中， 選殖與高溫逆境適應性有關之突變株， 分析突變株之生長發育情形與確認被突變的基因， 瞭解這些基因在水稻遇熱逆境時所扮演之角色與功能， 結果可做為水稻育種及改良熱耐性之參考；另方面計畫轉殖水稻第一族小分子量熱休克蛋白質（class I small heat shock proteins；sHSP-CIs）基因的轉錄股（ sense strand）與反轉錄股（ antisense strand） 片段， 即持續性表現或抑制熱休克蛋白質的表現量，藉此分析這群可受熱大量誘導合成及在種子形成初期出現的高保留性蛋白質對於水稻發育及稻米品質的影響。主要工作項目：（一）由T-DNA 插入基因水稻突變種的T2 種子篩選突變株， 篩選可耐異常高溫的水稻突變株和對高溫敏感的水稻突變株， 分析水稻中參與溫度調控機制功能的基因， 作為未來培育耐熱新品種水稻的依據。（ 二）根據已公佈的水稻突變株，搜尋已被確認為熱逆境相關基因突變之突變株， 分析植株的外部型態、耐熱性和稻米產量及品質， 所得結果同樣可作為未來選種及育種上的重要參考。（ 三）建立過量表現和抑制特定或全部sHSP-CIs 表現的水稻轉殖株，分析轉殖株與正常植株在生長發育上的差異情形、對環境逆境的耐性和水稻的產量與品質， 探討熱休克蛋白質是否參與控制水稻基本的生長發育與稻米形成過程。 Using functional genomics study, we are trying to screen thermo-tolerant lines from rice T-DNA insertion mutants or the established database of insertion rice mutants. Analysis of development and phenotype of mutants is being conducted to characterize the mutants. Besides, the mutated genes and the roles of these thermo-related genes are also identified. The results should benefit the establishing a base for rice breeding. On the other hand, to understand the physiological functions of rice class I small heat shock proteins (sHSP-CIs) for rice development and grain properties, we plan to transform sense strand and antisnese fragment of sHSP-CI genes into rice and characterize phenotype as well as yield of these transgenic rice plants. The three major works are as follows. Firstly, the work is to screen thermo-tolerant or -sensitive mutants from T2 seeds of rice T-DNA insertion mutants and identify the mutated genes as well as their roles on thermo-tolerance. The data obtained from here can provide us much information for breeding thermo-tolerant rice. Secondly, we try to search heat related gene mutant lines from database of the insertion mutant population. The phenotype, heat tolerance, grain yield, and grain properties of the rice mutants are analyzed and characterized. The results shown here are useful for improving thermo-resistance of rice. Finally, we plan to introduce sHSP-CI genes into rice to express sHSP-CIs constitutively and use RNA interference (RNAi) techniques to knock down the transcription of one or all sHSPs in rice at any conditions including normal temperature and heat stress. Assay for growth and development, tolerance to stresses, and rice grain yield and properties of these transgenic rice plants is being conducted to find the physiological function of sHSP-CIs on the development of rice grains. 研究期間：9601 ~ 9612
