Development of a label-free impedance biosensor for detection of antibody-antigen interactions based on a novel conductive linker

NCUIR > Engineering College > Department of Chemical and Materials Engineering > journal & Dissertation > Item 987654321/49929

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Title:	Development of a label-free impedance biosensor for detection of antibody-antigen interactions based on a novel conductive linker
Authors:	Chen,CS;Chang,KN;Chen,YH;Lee,CK;Lee,BYJ;Lee,ASY
Contributors:	化學工程與材料工程學系
Keywords:	POLARIZATION INTERFEROMETRIC BIOSENSOR;SURFACE-PLASMON RESONANCE;SELF-ASSEMBLED MONOLAYERS;GOLD ELECTRODES;BIOMOLECULAR INTERACTIONS;REAL-TIME;ELECTROCHEMICAL BIOSENSORS;DNA-SENSORS;SPECTROSCOPY;IMMUNOSENSOR
Date:	2011
Issue Date:	2012-03-27 16:26:43 (UTC+8)
Publisher:	國立中央大學
Abstract:	We developed a label-free impedance biosensor based on an innovative conductive linker for detecting antibody-antigen interactions. As the often used conventional long chain thiol is a poor conductor, it is not a suitable material for use in a faradaic biosensor. In this study, we adopted a thiophene-based conductive bio-linker to form a self-assembled monolayer and to immobilize the bio-molecules. We used cyclic voltammetry and impedance spectroscopy to verify the enhanced conductivity properties. Results showed that the electron transfer resistance of this new conductive linker was 3 orders of a magnitude lower than for a case using a conventional long chain thiol linker. With the decreased impedance (i.e. increased faradaic current), we can obtain a higher signal/noise ratio such that the detection limit is improved. Using fluorescence microscopy, we verified that our new conductive linker has a protein immobilization capability similar to a conventional long chain thiol linker. Also, using S100 proteins, we verified the protein interaction detection capability of our system. Our obtained results showed a linear dynamic range from 10 ng/ml to 10 mu g/ml and a detection limit of 10 ng/ml. With our new conductive linker, an electrochemical impedance biosensor shows great potential to be used for point-of-care applications. (C) 2010 Elsevier B.V. All rights reserved.
Relation:	BIOSENSORS & BIOELECTRONICS
Appears in Collections:	[Department of Chemical and Materials Engineering] journal & Dissertation

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