Green Tea Catechins: Inhibitors of Glycerol-3-Phosphate Dehydrogenase

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题名:	Green Tea Catechins: Inhibitors of Glycerol-3-Phosphate Dehydrogenase
作者:	Kao,CC;Wu,BT;Tsuei,YW;Shih,LJ;Kuo,YL;Kao,YH
贡献者:	生命科學系
关键词:	3T3-L1 CELLS;EPIGALLOCATECHIN GALLATE;POLYPHENOLS;(-)-EPIGALLOCATECHIN-3-GALLATE;ACCUMULATION;MECHANISMS;CAFFEINE;GENE
日期:	2010
上传时间:	2012-03-27 18:09:07 (UTC+8)
出版者:	國立中央大學
摘要:	Green tea catechins, especially (-)-epigallocatechin-3-gallate (EGCG), are known to regulate obesity and fat accumulation. We performed a kinetic analysis in a cell-free system to determine the mode of inhibition of glycerol-3-phosphate dehydrogenase (GPDH; EC 1.1.1.8) by EGCG. GPDH catalyzes the beta-nicotinamide adenine dinucleotide (NADH)-dependent reduction of dihydroxyacetone phosphate (DHAP) to yield glycerol-3-phosphate, which serves as one of the major precursors of triacylglycerols. We found that EGCG dose-dependently inhibited GPDH activity at a concentration of approximately 20 mu M for 50% inhibition. The IC(50) values of other green tea catechins, such as (-)-epicatechin, (-)-epicatechin-3-gallate, and (-)-epigallocatechin, were all above 100 mu M. This suggests a catechin type-dependent effect. Based on double-reciprocal plots of the kinetic data, EGCG was a noncompetitive inhibitor of the GPDH substrates, NADH and DHAP, with respective inhibition constants (Ki) of 18 and 31 mu M. Results of this study possibly support previous studies that EGCG mediates fat content.
關聯:	PLANTA MEDICA
显示于类别:	[生命科學系] 期刊論文

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