A simple method using Pyrosequencing (TM) to identify de novo SNPs in pooled DNA samples

NCUIR > college of Health Sciences and Technology > Department of Life Science > journal & Dissertation > Item 987654321/50784

Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/50784

Title:	A simple method using Pyrosequencing (TM) to identify de novo SNPs in pooled DNA samples
Authors:	Lin,YS;Liu,FGR;Wang,TY;Pan,CT;Chang,WT;Li,WH
Contributors:	生命科學系
Keywords:	ALLELE FREQUENCY ESTIMATION;SINGLE NUCLEOTIDE POLYMORPHISMS;COMPLEX HUMAN-DISEASES;CASE-CONTROL DESIGNS;LINKAGE DISEQUILIBRIUM;MUTATION DETECTION;SEQUENCING METHOD;RELATIVE POWER;TECHNOLOGY;QUANTIFICATION
Date:	2011
Issue Date:	2012-03-27 18:10:07 (UTC+8)
Publisher:	國立中央大學
Abstract:	A practical way to reduce the cost of surveying single-nucleotide polymorphism (SNP) in a large number of individuals is to measure the allele frequencies in pooled DNA samples. Pyrosequencing (TM) has been frequently used for this application because signals generated by this approach are proportional to the amount of DNA templates. The Pyrosequencing (TM) pyrogram is determined by the dispensing order of dNTPs, which is usually designed based on the known SNPs to avoid asynchronistic extensions of heterozygous sequences. Therefore, utilizing the pyrogram signals to identify de novo SNPs in DNA pools has never been undertook. Here, in this study we developed an algorithm to address this issue. With the sequence and pyrogram of the wild-type allele known in advance, we could use the pyrogram obtained from the pooled DNA sample to predict the sequence of the unknown mutant allele (de novo SNP) and estimate its allele frequency. Both computational simulation and experimental Pyrosequencing(TM) test results suggested that our method performs well. The web interface of our method is available at http://life.nctu.edu.tw/similar to yslin/PSM/.
Relation:	NUCLEIC ACIDS RESEARCH
Appears in Collections:	[Department of Life Science] journal & Dissertation

Files in This Item:

File	Description	Size	Format
index.html		0Kb	HTML	606	View/Open

社群 sharing

Loading...