Toona sinensis (leaf extracts) inhibit vascular endothelial growth factor (VEGF)-induced angiogenesis in vascular endothelial cells

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题名:	Toona sinensis (leaf extracts) inhibit vascular endothelial growth factor (VEGF)-induced angiogenesis in vascular endothelial cells
作者:	Hseu,YC;Chen,SC;Lin,WH;Hung,DZ;Lin,MK;Kuo,YH;Wang,MT;Cho,HJ;Wang,L;Yang,HL
贡献者:	生命科學系
关键词:	TUMOR-GROWTH;GALLIC ACID;MATRIX METALLOPROTEINASES;CANCER-CELLS;TYROSINE KINASE;ANTIOXIDANT;APOPTOSIS;INVASION;RECEPTOR;IDENTIFICATION
日期:	2011
上传时间:	2012-03-27 18:11:34 (UTC+8)
出版者:	國立中央大學
摘要:	Aim of the study: Toona sinensis is well known as a traditional Chinese medicine; also, it has been shown to exhibit anticancer and anti-inflammatory effects. This study was aimed at evaluating the anti-angiogenesis effect of the aqueous extracts of Toona sinensis (TS extracts) or gallic acid, a major component of IS extracts, against both VEGF-induced EA.hy 926 and human umbilical vein endothelial cells (HUVECs). Materials and methods: Anti-proliferative activity of IS extracts or gallic acid, was determined against EA.hy 926 and HUVECs by trypan blue exclusion method. Invasion, tube formation and chick chorioallantoic membrane assay were carried out to determine the in vitro and in vivo anti-angiogenic effects. Results: Non-cytotoxic concentration of IS extracts (50-100 mu g/mL) and gallic acid (5 mu g/mL) inhibited the proliferation of VEGF-stimulated EA.hy 926 and HUVECs. Inhibitory effects of IS extracts and gallic acid on angiogenesis were assessed by VEGF-induced migration/invasion and capillary-like tube formation by EA.hy 926 and HUVECs. Additionally, gelatin zymography assays showed that IS extracts and gallic acid suppressed the activity of metalloproteinase (MMP)-9 and MMP-2 activated by VEGF. In vivo, TS extracts and gallic acid strongly suppressed neovessel formation in the chorioallantoic membrane of chick embryos. Flow cytometry analyses and Western blot demonstrated that treatment with IS extracts and gallic acid induced Go/G, arrest in VEGF-stimulated EA.hy 926 cells via a reduction in the amounts of cyclin D1, cyclin E, CDK4, hyperphosphorylated retinoblastoma protein (pRb). VEGFR-2, and eNOS. Conclusions: These results support an anti-angiogenic activity of Toona sinensis that may contribute critically to its cancer and inflammation chemopreventive potentials. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
關聯:	JOURNAL OF ETHNOPHARMACOLOGY
显示于类别:	[生命科學系] 期刊論文

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