探討光對Penicillium brevicompactum在液態發酵中生產Mycophenolic acid之影響及新型LED光反應器之設計;Effects of Light on the Production of Mycophenolic Acid by Penicillium brevicompactum in Batch Cultures and Novel Design of LED Photobioreactor

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Title:	探討光對Penicillium brevicompactum在液態發酵中生產Mycophenolic acid之影響及新型LED光反應器之設計;Effects of Light on the Production of Mycophenolic Acid by Penicillium brevicompactum in Batch Cultures and Novel Design of LED Photobioreactor
Authors:	徐敬衡
Contributors:	中央大學化學工程與材料工程學系
Keywords:	化學工程類;青霉菌(Penicillium brevicompactum);麥考酚酸(Mycophenolic acid);光(light);LED 光生物反應器;Penicillium brevicompactum;mycophenolic acid;light;photo-bioreactor;lightemitting diode (LED)
Date:	2008-09-01
Issue Date:	2012-10-01 11:15:52 (UTC+8)
Publisher:	行政院國家科學委員會
Abstract:	MPA 在生化合成（biosynthesis）主要是經由兩條路徑polyketide pathway 和 isoprenoid pathway 的二次代謝產物。一般來說，利用環境因子的改變，可以促進菌體的生長，藉由光強度及光波長對於代謝型態的改變而提高產率，並且設計新型LED 光反應器降低操作成本也為其主要優勢。在一般微生物發酵中，最多人探討的因子無非是溫度、pH 值、通氣量、培養基組成份等，對於「光源」之研究，其對象不外乎是具有葉綠素之植物細胞或具有菌綠素之光合菌等。然而，近年來隨著基因工程的發展，科學家逐漸了解微生物體內的一些機制，例如合成蛋白質、繁殖的方式等等；於是乎，不少學者開始探討光與微生物之間的關連性。近年來又發現了某些真菌裡面擁有光的接收器，對於不同顏色的光會有不同的反應。例如，Aspergillus nidulans 是一株真菌，並不屬於光合菌，但照光的確會改變其生長情形。由此進一步證實真菌其實也會有光接受器的存在。由於Aspergillus nidulans 和Penicillium brevicompactum 的外觀及生長模式都非常相似，均屬子囊真菌，因此推測P. brevicompactum 或許也有類似的接收器。本計畫所研究預設為兩年計畫，主題乃是利用光強度及光波長對於Penicillium brevicompactum 生產MPA 之影響，以及新型真菌光反應器設計，研究方向如下所述： 1. 利用LED 光源特性探討光對真菌之研究並進行調控：透過不同光強度、不同光波長對特定真菌Penicillium brevi-compactum 生長與產物代謝之影響。由於不同顏色之LED 光源，具有特定之波長。尤其是二次代謝物，往往是在菌體停止生長才會產生，其最適條件往往不同於生長條件。本研究擬尋找到適合生長與產物生成各自之最佳條件，例如某特定波長與強度將利於菌體的生長，但是某特定波長與強度將利於產物的生成。 2. 以真菌對光之特殊需求為考量設計之LED 光生物反應器：透過本尋找到適合生長與產物生成各自之最佳條件，本研究擬設計出符合真菌對光之特殊需求之光反應器。LED 燈具有低耗能、高亮度、窄頻、體積小及壽命長等特性，對於微生物光反應器而言此乃最合適之光源，因此，利用條鍊式LED 燈作為光源不僅方便更換不同光波長之燈源也利於控制光強度，混合型光波長更可以因應不同的需求而搭配，不僅僅只是針對單一菌種而設計，可以因著各個菌種而改變。本研究之創新在於： 1. 建立國際上首次探討光對真菌Penicillium 之發酵相關的基礎研究。 2. 建立國際上首次探討兩階段LED 光生物反應器於Penicillium 之研究。 3. 建立以Penicillium為model 之LED光生物反應器，供抗生素工業之參考。 ; MPA biosynthesis involves two of the major pathways for secondary metabolite formation of the polyketide and isoprenoid. In general, environmental factors can enhance fungal growth and modify the formation of metabolism resulting in high productivity. However, previous light research focused majorly on chlorophyll containing cells such as plant and algae or photosynthetic cells but little research focus on cells without chlorophyll such as fungi. Recently, different photo-receptors and related proteins such as phytochrome, opsin, and crytochrome have been purified and identified from the cells without chlorophyll including fungi. However, little information is revealed in literature regarding the engineering aspects including the optimal light wavelength and light intensity on fungal growth and product formation. LED photo-bioreactors have been proposed by several researchers; however, the design of the photo-bioreactor is limited to the chlorophyll containing cells which may not be proper toward the non-chlorophyll containing cells such as fungi. Thus, it is essential to explore the novel design considering the difference of photo-receptors of fungi. Our project is a two-year research proposal which will clarify the influence of light on fungal growth and its product formation and related photobioreactor design as follows: 1. Influence of specific light wavelength and light intensity on the cell growth and MPA production of Penicillium brevi-compactum. The optimal light conditions for cell growth and product formation will be obtained using LED light bulbs in the flask cultures. 2. Design of the novel LED photo-bioreactors: strings of LED light bulbs will be used to construct a 2-Liter air-lift or bubble column photo-bioreactors. 3. Construct a two-stage light control operation strategy for the fungi in the novel LED photo-bioreactor. The novelty of the proposal are listed as follows: 1. To provide the first hand information about the light influence on the growth and product formation of Penicillium brevicompactum. 2. To construct the first two-stage light control operation strategy for fungi in the novel LED photo-bioreactor. 3. To provide an essential scientific reference of antibiotic industry using fungi as antibiotics producers. ; 研究期間 9708 ~ 9807
Relation:	財團法人國家實驗研究院科技政策研究與資訊中心
Appears in Collections:	[Department of Chemical and Materials Engineering] Research Project

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