由於當今社會產業結構的改變，從業人口長時間處於室內環境工作，在室內和半開放性空間中人員經常出入移動下易使空氣品質不良。而依據美國環保署研究報告指出，室內生物氣膠濃度往往比戶外的濃度還高。而近來流行病學相關研究也已指出空氣中生物氣膠的濃度，與人體的許多症狀與生物氣膠的曝露程度有關，也反映了室內生物氣膠濃度為健康風險的一個指標性因子。此外，多篇報告也探討室內生物氣膠濃度與病態大樓症候群亦有諸多關聯，因此室內空氣中的生物氣膠濃度已成環保署推動相關法規的重點項目之一。 本研究以中央大學國鼎光電大樓奈米生物醫學實驗室做為採樣地點。使用重力沉降法採集研究室五個地點的微生物，三個時段，每個時段採樣時間為四小時，以採集配對研究生實驗室的空氣樣本，並培養三天後，才進行菌落數分析以估算生物氣膠的濃度。藉由比較分析以冀求影響生物氣膠濃度高低差異之各種因子，從而得知影響生物氣膠濃度的主導因子。 一般生物實驗較常使用的菌落測數方法為直接測數法(Direct count)，但對肉眼較為吃力。故本研究採用影像二值化和濁度測定法(Turbidity measurement)分析，提升生物氣膠量的評估準確性。在本系統之建立基礎下，希望能提供人員自主管理之用，採取必要的防護措施和消毒作業，有助於室內空氣品質的改善和管制策略。 Due to the changes in social structure of nowadays, employees in the indoor environment for a long time. People who frequent indoor and half-open space make the indoor air worse. According to the U.S. Environmental Protection Agency study reported that indoor bioaerosol concentrations are often higher than outdoor concentrations. Recent epidemiological research has also pointed out that the degree of the bioaerosol exposure to air is related to human’s symptoms, also reflects the indoor bioaerosol concentration of an indicator of health risk factor. In addition, the report also explored the indoor bioaerosol concentration correlate with sick building syndrome. the indoor air concentration of bioaerosols has become one of the key projects that Environmental Protection Agency promote the relevant laws and regulations. In this study, Nation Central University Guo-ding photoelectric building Nano Biomedical Laboratory as sampling locations.The air microorganism of laboratory are collected by using gravity sedimentation at five laboratory locations, three periods, each period is four hours . We also cultivate three days later, we analyze the number of colonies to estimate bioaerosol concentration. By comparing the analysis to get what factors affect the difference of bioaerosol concentration. It’s in order to find the dominant influences on bioaerosol concentration. The common biological experiments methods for measuring number of colonies is direct count, but it is more difficult for the naked eye. herefore, we used binarized image analysis and turbidity measurement to improve the accuracy of bioaerosol concentration . In this system, the establishment of foundation, hoping to provide workers self-management purposes, take the necessary protective measures and disinfection operations,. And It’s contribute to the improvement of indoor air quality and control strategies.
