Polymethylmethacrylate (PMMA) 被當作一種玻璃的替代材料，由於其透光度高、化學性質穩定、具有熱塑性而且價格不貴的特性，讓他適合應用於生物醫學領域。相較於各種不同的製程，如凝膠化、射出成型和鑄造，電漿聚合方法是唯一能均勻且快速的合成出達到奈米等級的PMMA薄膜。在這篇研究中，我們探討在真空腔體中以電漿聚合沉積在玻璃基板上的PMMA薄膜，其結構、成分、表面和化學性質。在製程部分，製作出在不同RF功率、工作壓力和沉積時間之下的PMMA薄膜。在材料檢測的部分，將進行以下的性質測量：使用Surface Profiler進行膜厚測量(50~200 nm)；以FT-IR進行微結構分析；XPS測量其表面化學成分；使用AFM掃描薄膜的表面形貌和粗糙度；最後以接觸角量測儀測量表面親水性。初步結果顯示了沉積的PMMA薄膜其物理、化學性質在一周期間內皆很穩定，而此研究將PMMA薄膜投入細胞培養的實驗中，進行了人類肝臟細胞的增生性能、細胞活性和其功能執行的分析。 Polymethylmethacrylate (PMMA), also called acrylic glass, is transparent, chemically stable, thermoplastic and inexpensive, making it suitable for many biochemical applications. Among different fabrication processes of PMMA such as gelation, injection and casting, plasma polymerization is the one capable of depositing nano scale films on almost any substrate uniformly and rapidly. In this study, we investigated the structure, composition, surface and mechanical properties of deposited PMMA films on glass by RF power plasma inside a vacuum chamber. The deposition was carried out under different RF power, working pressure and deposition time, from which an optimal fabrication condition was explored. On the characterization part, following tests were carefully conducted: the thickness (around 50 to 200nm) was estimated by surface profiler microstructures was determined by Fourier transform infrared spectroscopy (FT-IR) surface chemical compositions were examined by X-ray photoelectron spectroscopy (XPS) surface morphology and roughness were measured by atomic force microscopy (AFM) and the wettability by water contact angle. Primary results showed that deposited films are physically and chemically stable for more than a week, which are readily available for hepatocytes cell culture, cell proliferation, cell activity and function execution test.
