本篇論文分成兩個部分： 第一部分，我們實驗室成功地在具有羧酸官能基的中孔洞材料表面修飾上可調節環狀大小之中孔洞材料，命名為XC-CAR-10，X代表雙端胺基化合物中間的碳數，應用於金屬吸附。大環分子效應對於提升環狀中孔洞材料的吸附能力扮演著相當重要的腳色，特別是3C-CAR-10，對於鉛離子具有相當優異的吸附能力和選擇性，飽和吸附能力約為300 ?g/mg。其具有最適合的幾何大小及孔洞可與金屬陽離子形成主-客系統。 第二部分，為了開發蛋白質純化的新穎材料，我們企圖將具有內環狀結構的中孔洞材料應用於固定化金屬親和層析法中，作為純化蛋白質的工具。由於環狀結構的中孔洞材料為相當優異的金屬吸附劑，吸附鈷 (II) 和鎳 (II) 離子後，形成金屬-環狀中孔洞材料錯合物展現出對於組胺酸標籤的蛋白質有相當不錯的鍵結能力，因此為蛋白質純化材料之發展打開了新的一頁。 Two parts in this thesis: Part one: annular rings with controllable sizes are successfully created on the surface of COOH-functionalized mesoporous silica (namely, XC-CAR-10, where X represents the number of carbons in the diamines used) for the adsorption of metal ions. Macrocylic effect in our annulated mesoporous silica plays a vital role in enhancing its adsorption ability for meta ions. In particular, excellent adsorption ability and selectivity towards ~300 ?g/mg of Pb2+ metal ion were achieved using 3C-CAR-10. 3C-CAR-10 contains suitable geometric sizes and the cavities offer the “best-fit” for the cations for the formation of host-guest complexes. Part two: in order to develop novel materials for protein purification, we performed annular mesoporous silica on immobilized metal affinity chromatography as a tool for protein purification. Because the annular mesoporous silica is an excellent metal ion adsorbent, e.g. Co2+ and Ni2+, the M2+-annulated materials complexes were applied for the assays of protein purification. Finally, annulated mesoporous silica showed higher binding capability of proteins with his-tags. Thus, it can open an auxiliary avenue in the development of a new resin for the protein purification process.
