環境逆境下水稻熱休克轉錄因子差異性調控機制分析;Analysis for the Mechanism of Differential Regulation of Rice Heat Shock Transcription Factors under Environmental Stress

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Title:	環境逆境下水稻熱休克轉錄因子差異性調控機制分析;Analysis for the Mechanism of Differential Regulation of Rice Heat Shock Transcription Factors under Environmental Stress
Authors:	葉靖輝;吳少傑
Contributors:	國立中央大學生命科學研究所
Keywords:	農藝;生物技術(農)
Date:	2013-12-01
Issue Date:	2014-03-17 11:49:15 (UTC+8)
Publisher:	行政院國家科學委員會
Abstract:	研究期間：10208~10307;Our previous studies have shown that the expression profiles of rice class I small heat shock protein (sHSP-CIs) genes were diversified in response to heat and azetidine (AZC) stresses. We have found that an AZRE (Azetidine responsive element; GTCCTGGACG), localized on the promoter regions of Oshsp17.3 and Oshsp18.0 genes, was associated with OshsfA4b and heat shock elements (HSEs) to regulate heat shock response and AZC-induced cytosolic protein response. However, OshsfA4b and AZRE did not involve in the induction of Oshsp16.9A gene under heat stress. In this three-year project, our major goal is to further study the differential regulatory mechanism for heat shock factors (HSFs) on the induction of sHSP-CIs genes. Firstly, using the expression level of Oshsp17.3::GUS as an indicator, we are trying to analyze the effects of AZRE and HSEs on the HSF-regulated expression of sHSP-CI genes. Secondly, we plan to screen and characterize other rice HSFs and Arabidopsis HSFs specific for AZC-induced expression of sHSP-CI genes. Thirdly, we plan to introduce AZRE and HSE into rice genome and establish transgenic rice plants, which are used to study the differential regulation of HSFs. Fourthly, we try to use EMSA (Electrophoretic Mobility Shift Assay) technique to analyze the interaction between specific HSFs and AZRE and HSE. Finally, we plan to compare tunicamycin-induced UPR (unfolded protein response) with AZC-induced CPR and UPR.
Relation:	財團法人國家實驗研究院科技政策研究與資訊中心
Appears in Collections:	[Graduate Institute of Life Science] Research Project

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