| 摘要: | 研究期間：10108~10212;The purpose of this study is to produce biogas energy using combinations of agricultural wastes and animal manure produced in Taiwan. It is anticipated to be operated by a design called anaerobic co-digestion, particularly, a solid-state fermentation containing more than 20% of total solid will be used. Considering that different pig farmers have their own feed formula in Taiwan livestock industry, the intestinal microbiota of pigs would be different from other countries. In addition to manure from local farmers, this project is aimed to use different pre-treated rice straw which has been developed by Institute of Nuclear Energy Research, Taiwan. The use of pretreated rice straw for the improvement of cellulosic degradation has been demonstrated in pilot-scale studies for the bioethanol production. Therefore, the explosure of cellulose in rice straw after pretreatment has been proved to be helpful for the conversion of cellulose to ethanol. In the microbial community study, our previous results also demonstrated that cellulolytic microbes and enzymes are dominant in the lab-scale anaerobic digestion using manure as a sole feed stock. It is widely accepted that the hydrolytic activity from bacteria is the bottleneck of biogas production when using organic solid matters as feed stock. The microbes and their community structure from a co-digestion system containing both manure and agricultural wastes (most are rich in lignin and cellulose) are even more complicated and, therefore, relatively unknown. Thus, the manipulation of the ratio of the organic solid wastes and the optimization of the operation conditions are crucial for an effective bioconversion process. There are three sub-projects in this joint project: 1) Biogas production from direct anaerobic digestion of agricultural solid wastes (Chu-Ching Lin, PI, Kuen-Sheng Wang, co-PI and Dyi-Hwa Tseng, co-PI): The operation will be optimized at the lab-scale for the first year and to be scaled-up at the second year. 2) Microbial community and bioresource in solid-state anaerobic co-digestion (Shir-Ly Huang, PI and Chu-Ching Lin, co-PI): To study the community structure (both bacteria and archea) and indicator genes (two for hydrolysis of organic wastes and two for methangenesis) during the solid-state anaerobic co-digestion. The cellulolytic microbial and enzymes isolated from our system can be further characterized for the applications in biomass processing industries in the second year. 3) Study of lignocellulosic material to improve biogas production (Wen-Song Hwang, PI, Gia-Luen Guo, co-PI and Wen-Hua Chen, co-PI): The sub-project dedicated to establish the pretreatment technology of lignocellulosic materials to improve biogas production in solid-state co-digestion system. For the second year, the test-run and scale-up research for solid-state co-digestion will be proformed. The energy efficiency will also be evaluated for this biogas technology. These results should provide helpful information for the commercialization of biogas production. After all, the goal of this joint project reaches a multiple wins including the reduction of agricultural wastes and global warming gases and the production of biomass energy. |
