砷化合物廣泛的存在於水、土壤以及空氣中。在流行病學的 統計上，慢性的砷暴露和一些疾病的發生有密切關係，如：皮膚 癌、肝癌、膀胱癌、肺癌以及烏腳病等等。在本實驗室前人的研 究中顯示HeLa S3 細胞經過5 μM 亞砷酸鈉處理，會有大約35 % 的細胞停置在有絲分裂中期，而處理staurosporine （蛋白質激酉每 抑制劑）之後，會促使中期停滯細胞離開有絲分裂期。在本研究 中，我們試著觀察亞砷酸鈉所誘導下來的中期停滯細胞經過 staurosporine 作用後，其顯微結構的變化以及蛋白質的修飾作 用。結果顯示亞砷酸鈉所誘引下來的細胞經過staurosporine 處理 之後，染色體以及拉長與濃染的紡綞體會解構、核膜會開始再形 成且會產生多核的現象。除此之外，藉由二維電泳的分析，我們 也觀察到亞砷酸鈉誘引下來的細胞，經過staurosporine 處理後， 其蛋白質表現的改變，然而，這些蛋白質表現的變化仍需要經過 MALDI-TOF 或是二維電泳-西方點墨法做進一步確認。 Arsenical compounds are widely distributed in the water, soil and air. Epidemiological evidences have revealed that arsenic exposure is associated with increased incidences of a variety of diseases, such as Blackfoot Disease, and skin, liver, bladder and lung cancers. Previous studies in our laboratory have shown that treatment of HeLa S3 cells with 5 μM arsenite leads to about 35% of the cells arrested in the mitotic metaphase. Treatment of arsenite-arrested mitotic metaphase cells with staurosporine (protein kinase inhibitor) enforces them exiting from mitosis. In this study, we try to observe changes of ultrastructure and investigate protein modifications by staurosporine on arsenite-arrested mitotic cells. Our results show that treatment of arsenite-arrested mitotic cells with staurosporine leads to dissolution of chromosome and elongated and condensed spindle, reformation of nuclear envelope and generation of multiple nuclei. In addition, proteins modified by staurosporine treatment on arsenite-arrested mitotic cells are also observed by two-dimentional gel electrophoretic analysis. However, these proteins should be further identified and characterized by MALDI-TOF or 2D-Western techniques.
