研究期間：10108~10207;This proposed research is a continuing work from previous year’s projects (2010/04/01 ‐ 2011/04/31, Cell Traction Forces on a Viscoelastic Gel (NSC 99‐2218‐E‐008‐006) 2010/08/01 ‐ 2012/07/31, Cell Traction Forces on a Viscoelastic Gel ‐ Chemotaxis and Focal Adhesion (NSC 100‐2221‐E‐008 ‐057)). Our main goals are aimed to design and test a novel experimental device for the chemotaxis of placenta derived multi‐potent cells (PDMCs). The overall approach combines simulation and experiment to give a quantitative correlation among cell attachment, migration, mechanical responses of substratum and chemical diffusion in chemotaxis. Following issues will be the focuses of this study: chemically fabrication of gelatin and alginate substratum; surface morphology measurement; measurement for thermal and mechanical properties of gelatin and alginate substrata; design of chemotaxis experiment; establishment of PDMC cell‐culture procedure; the measurement and quantitative characterization of cell migration under chemotaxis; additional test only if time allows: protein electrophoresis (Sodium Dodecyl Sulfate, SDS‐PAGE) for PDMCs underwent chemotaxis. This proposal also aims to design a simple device for chemotaxis test which is a important biophysical phenomenon in many physiological activities such as motility, migration, phagocytosis, oxygen and carbon dioxide exchange, thrombosis/fibrinolysis, inflammation and tumor angiogenesis etc. Our approach will combine two continuous developments in the mechanical characterizations on mammalian tissues and hydrogels, cellular deformation through collaborations with biomedical research groups in hospitals. A general prototype approach of the characterizing technique has been developed to investigate several biological and soft hydrogel substratum with proved feasibility. In parallel, a numerical modeling by the PI has been developed based on the theory of hyperelasticity in order to simulate the experimental work and comprehend the material responses of hydrogels in the virtue world by correlating with the experimental measures. These studies are crucial for the future design of chemotaxis test because the coupling of focal adhesion and chemotaxis are one of the first and foremost mechanism for the differentiation, migration and apoptosis throughout the life cycle of cells. In addition, under permitted circumstances, a smaller portion of this proposal shall be protein analysis by polymerase chain reactions (PCR) in an attempt to decipher the relation between adhesion, trans‐membrane proteins and chemotaxis induced migration.
