蓮花為多年生水生植物 (真雙子葉植物；龍眼目；蓮科；蓮屬)，主要分布在亞洲的熱帶和亞熱帶地區。它的葉子具有蓮葉效應因此有自我清潔的能力。到目前為止，已經發現了一些寄生真菌，如Alternaria nelumbii, Colletotrichum gloeosporioides, Pseudocercospora nymphaeacea，但是內生真菌尚未有任何記錄、發現。本研究的目的是探測和鑑定真菌，包括外部真菌和內生真菌。我們在台灣不同的地方採集蓮花葉片，每一片葉子分為葉片和葉柄。將植物片段用95%乙醇和6%次氯酸鈉進行表面消毒，再用無菌水清洗。表面消毒的有效性用imprint technique來驗證。從約1002個植物片段中獲得了476株菌株，可被分為33個種類。大多數菌株由葉片分離出，佔71％，而葉柄只有29％。從植物組織中分離的內生真菌利用菌種型態顯微鏡觀察以及DNA分析來鑑定種類。其中外生真菌Pseudocercospora sp. nov. 和Dissoconium sp. nov. 為新種類。; Nelumbo nucifera is an herbaceous, perennial aquatic plant belonging to Nelumbonaceae (order Proteales, in the clade eudicots, genus Nelumbo), and is found in tropical and subtropical Asia. The self-cleansing property of its leaves is called lotus effect. So far, some parasitic fungi of N. nucifera have been reported, like Alternaria nelumbii, Colletotrichum gloeosporioides, Pseudocercospora nymphaeacea, but the endophytic fungi have not been recorded. The purpose of this study is to detect and identify fungi from leaves of N. nucifera including ectophytic and endophytic fungi. We collected leaves of N. nucifera at different places in Taiwan. Each leaf was divided into leaf lamina and petiole. These parts were surface-sterilized with 95% ethanol and 6% sodium hypochlorite and then rinsed in sterile water. The effectiveness of surface sterilization was verified by the imprint technique. From 1002 plant segments, 476 isolates were produced in culture, comprising 33 typical terrestrial species. Most isolates were from leaves (71%) compared to those from petiole (29%). Among ectophytic fungi, the species Pseudocercospora sp. nov. and the genus Dissoconium are recorded for Taiwan for the first time.
