本研究目標要發展一系列光學檢測微流道系統(series of optical biosensor in micro-fluidic system) 置入具有微型化、表面奈微級結構的光學元件作為一基因快速檢測平台：GMR(guided-mode resonance)，本元件利用半導體技術，製作一次波長光柵結構，並利用其產生的窄帶高反射率之效果，及其光柵表面區域之高敏感度，應用於共種生化反應，並設計一系列表面改質於晶片表面，使生物分子以共價鍵鍵結固定於晶片表面,並成功的以此光學即時量測系統即時監測整個反應過程，觀測是否有雜交反應。本光學式檢測系統具有以下優點：免標定(label free)、微型化(mini size)、可高通量(high through put)、易與其它半導體元件接合、高訊雜比(high quality)、高穩定性、即時偵測(real time)之優點。We used the guided-mode resonance (GMR) device to achieve the real-time detection on DNA hybridization process. The bulk-shift sensitivity is about 200 (nm/RIU) of the biosensor in the sandwich types of micro-fluidic system, and the detection limit is approximately 10-5 refraction index unit (RIU). We proposed a novel method to detect the different length of DNA hybridizations of DNA attached the chip surface respectively. Obviously, this optical biosensor shows its promising role in developing a real-time testing system which is with the most benefits of label free, high throughput, small size, and high sensitivity, etc.
