從人類皮膚微生物總體中鑑定溶解磷酸鈣的細菌;Identification of Calcium Phosphate-degrading Bacteria from Human Skin Microbiome.

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Title:	從人類皮膚微生物總體中鑑定溶解磷酸鈣的細菌;Identification of Calcium Phosphate-degrading Bacteria from Human Skin Microbiome.
Authors:	王淞民;Wang,Sung-Min
Contributors:	生命科學系
Keywords:	微生物總體;皮膚;磷酸鈣;發酵;短鏈脂肪酸
Date:	2016-07-27
Issue Date:	2016-10-13 13:03:45 (UTC+8)
Publisher:	國立中央大學
Abstract:	磷酸鈣在人體上過的累積會導致不同類型的疾病產生，像是結石以及皮膚搔癢等。我們的研究主要是研究皮膚微生物對於磷酸鈣結石的影響，我們利用含有豐富磷酸鈣的培養基從人類皮膚上鑑定出一株具有降解磷酸鈣的液化澱粉芽孢桿菌，細菌可以利用葡萄糖進行發酵反應產生短鏈脂肪酸，進而降解磷酸鈣。秀麗隱桿線蟲已經被證實是研究許多生物過程一個非常好的動物模型，在此我們用它做為模型來探討液化澱粉芽孢桿菌的發酵液對於磷酸鈣的分解，透過餵食已用Alizarin red S (ARS) 染色後的磷酸鈣給線蟲，並加入發酵液與線蟲共同培養。我們發現發酵液 (包含短鏈脂肪酸) 可以有效的降解線蟲體內的磷酸鈣，其中醋酸的降解活性已經在小鼠中獲得證實。在本實驗中我們鑑定出一株能夠做為益生菌的液化澱粉芽孢桿菌，此外該菌株的發酵液能夠有效的降解磷酸鈣。;The accumulation of calcium phosphate stones in humans is a modern common disease of civilization, and contributes to many different types of stones-associated diseases. Our research has been mainly aimed to study the probiotic effects of skin bacteria on the degradation of calcium phosphate stones. We have identified a Bacillus amyloliquefaciens bacterium from the human skin by selecting from calcium phosphate rich agar plates in conjunction with 16S rRNA sequencing. The bacteria can ferment the glucose to short-chain fatty acids (SCFAs) which efficiently dissolve the calcium phosphate. Caenorhabditis elegans (C. elegans), proven to be an excellent model for the study of many biological processes, has been used as one of models to study the degradation of calcium phosphate by fermentation media of Bacillus amyloliquefaciens. C. elegans was fed with Alizarin red S-stained calcium phosphate particles along with/without the fermentation media. We found that the fermentation media containing SCFAs can degrade calcium phosphate in C. elegans. The degradation activity of acetic acid, one of SCFAs, was verified in mice. The novelty in our study includes the identification of a Bacillus amyloliquefaciens as a probiotic bacterium. Furthermore, the fermentation media of this bacteria can effectively degrade calcium phosphate.
Appears in Collections:	[Graduate Institute of Life Science] Electronic Thesis & Dissertation

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