建立人類皮膚益生微生物菌組銀行;Establishment of a human skin probiotic microbiome bank

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Title:	建立人類皮膚益生微生物菌組銀行;Establishment of a human skin probiotic microbiome bank
Authors:	馮健榮;Feng,Jian-Jung
Contributors:	生命科學系
Keywords:	微生物總體;益生菌;慢性腎臟病;痤瘡;Microbiome;Probiotic;Chronic renal disease;Acne
Date:	2016-08-26
Issue Date:	2016-10-13 13:04:42 (UTC+8)
Publisher:	國立中央大學
Abstract:	人類皮膚微生物菌相失衡已被證實與許多皮膚疾病有關。目前主要分析皮膚上菌相的方法為價格昂貴的次世代定序。我們已經從皮膚上分離許多細菌並用16S rRNA序列來鑑定出其學名，並經由實驗找出其具有利用多種不同碳源進行發酵的活性。這些具有發酵活性的細菌我們定義其為益生菌，並儲存在我們的實驗室作為”皮膚益生菌銀行”。為了定量人體血液中自然產生之皮膚菌抗體，我們已經開發出一種免疫色層快篩試紙：由吸收墊、硝化纖維膜、結合上 Protein A 的奈米金和樣品墊組成。在硝化纖維膜上有實驗組區和控制組區分別點上細菌加熱破膜菌液和抗人類免疫球蛋白G二級抗體。在人類血液中，我們已成功使用免疫色層快篩試紙偵測到在皮膚菌中數量最多的痤瘡桿菌之抗體，顯示這種快篩試紙半定量人類皮膚微生物菌群抗體的可行性。;The dysbiosis of the human skin microbiome has been associated with many skin disorders. The next generation sequencing (NGS), although it is cost-effective, is a major method to analyze the dysbiotic skin microbiome. We have isolated many skin bacteria, identified them by 16S rRNA sequencing and tested their fermentation activities using various carbon sources. The fermenting bacteria are defined as probiotic bacteria and stored in our laboratory as a “Skin Probiotic Bank”. To quantify the antibodies to skin bacteria naturally produced in human blood, we have contracted an immunochromatographic test strip consisting of absorbent pad, nitrocellulose (NC) membrane, protein A -conjugated gold nanoparticle pad and sample pad. Test and control zones on NC membranes are created by spotting the heat-killed lysates of bacteria and anti-human IgG secondary antibody. In the human blood, we are able to detect the antibodies to Propionibacterium acnes, a most abundant bacterium in human skin, using immunochromatographic test strips, demonstrating the feasibility of the test strips for semi-quantitatively profiling the natural antibodies to the huamn skin microbiome.
Appears in Collections:	[Graduate Institute of Life Science] Electronic Thesis & Dissertation

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