The Effects of Sustain Stretching and Compression in the Inter-beat Interval and Beat Rate Variability of Embryonic Cardiomyocytes

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題名:	The Effects of Sustain Stretching and Compression in the Inter-beat Interval and Beat Rate Variability of Embryonic Cardiomyocytes
作者:	柯奕恩;Estayan, Mhar Ian Cua
貢獻者:	生物物理研究所
關鍵詞:	心率;壓縮;Mechanical Stretching;Compression;Cardiomyocytes;Beat Rate Variability;Inter-beat Interval
日期:	2017-04-27
上傳時間:	2017-05-05 17:13:27 (UTC+8)
出版者:	國立中央大學
摘要:	許多的觀察指出心肺復甦術是在心臟上施加機械應力而使得心臟重新跳動。構成心臟的細胞主要有心肌細胞以及纖維母細胞兩種細胞都具有感應機械應力的離子通道並且可以被釓所抑制這項研究的目的是想要觀察在不同的密度的纖維母細胞的心肌細胞培養中，機械應力是否影響心肌細胞的動態反應。為了更詳細瞭解機械應力對心臟的動態的影響，我們設計了兩種機械應力的刺激方式: 1.) 持續性拉伸以及 2.)持續性壓縮。我們記錄了心肌細胞跳動的影響並且計算了的脈衝間隔以及心律變異。我們的研究發現機械應力對於有高濃度的纖維母細胞的心肌培養之脈衝間隔以及心律變異沒有顯著性影響，加入釓亦無影響。在攝氏27度的時候，機械應力可以影響低濃度的纖維母細胞的心肌培養之脈衝間隔以及心律變異。;Mechanical stress on the heart has been observed to retrieve heart beating by means of performing cardiopulmonary resuscitation. Cardiomyocyte and Fibroblast are cells that comprises the heart. Both cells have stretch activated ion channels which can be blocked by Gadolinium. The purpose of this experiment is to observe if mechanical stress affects the cardiac dynamics in cellular level and to investigate the different densities of fibroblast. To understand the cardiac dynamics under mechanical stress, we designed two types of mechanical stimulation: 1.) Sustain Stretching and 2.) Compression. Percentage change in the Inter-Beat Interval (IBI) and Beat Rate Variability (BRV) were computed. The result of this experiment shows that mechanical stretch does not affect the IBI and BRV for samples with high number of fibroblast. Gadolinium does not affect IBI and BRV when drug is added. Mechanical stress at 27˚C affects BRV and mechanical stress of low density fibroblast affects the IBI and BRV.
顯示於類別:	[生物物理研究所 ] 博碩士論文

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