設計並建構一全氟碳光生物反應器組用於分離混合氣體中之二氧化碳並同時提升微藻養殖及其經濟產物生成之效能;Design and Establishment of a Synthetic Perfluorocarbon Photobioreactor System for Carbon Dioxide Separation and Enhancement of Microalgal Growth and Productions

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Title:	設計並建構一全氟碳光生物反應器組用於分離混合氣體中之二氧化碳並同時提升微藻養殖及其經濟產物生成之效能;Design and Establishment of a Synthetic Perfluorocarbon Photobioreactor System for Carbon Dioxide Separation and Enhancement of Microalgal Growth and Productions
Authors:	孫仁厚;Sun, Jen-Hou
Contributors:	生醫科學與工程學系
Keywords:	擬球藻;光生物反應器;全氟碳化物;生物質;生物脂質;二氧化碳分離;氧氣收集;Microalgae;Photobioreactor;Perfluorocarbon;Biolipid;CO2 separation;oxygen collection
Date:	2017-11-28
Issue Date:	2018-01-16 10:22:50 (UTC+8)
Publisher:	國立中央大學
Abstract:	隨著溫室效應之日益受到重視，如何降低碳排放以及去除大氣層中過高比例的CO2已成為國際間重視的環保議題。為了有效去除混合氣體中的CO2並同時達成經濟產值的提升，本研究建構出一以全氟碳化物(Perfluorocarbon；PFC)為媒介之光生物反應器組(Perfluorinated Photobioreactor System；PPBRS)。本系統利用PFC優異的CO2吸收能力進行分離混合氣體中的CO2，並將PFC所吸收到的CO2以適當比例(v/v)輸送至擬球藻生長的光生物反應器瓶中，而擬球藻行光合作用所產生出O2再藉由PFC的傳遞導入另一單元中作收集。首先在單獨給予擬球藻不同濃度CO2並研究何種濃度為最適合擬球藻生長濃度實驗中發現，2% CO2的成長優於其他濃度組別(1 - 12%)，經與對照組(純打入空氣之組別)比較後發現，10天內的擬球藻細胞濃度多了2倍，生物質(Biomass)提高了1.25倍、總脂質(Total lipid)提高了1.57倍而二十碳五烯酸(eicosapentaenoic acid，EPA)提高了1.37倍。之後在實際於PPBRS全系統操作10天中，我們以60% N2 - 40% CO2作為模擬混合氣體並將其輸入至含有PFC的氣體純化分離單元，其吸附CO2的PFC再搭配吸附N2的PFC使其含有2% (v/v)的二氧化碳比例來供給擬球藻生長。結果顯示，PPBRS能在10天的操作下，有效的分離出混合氣體中的N2並其內的CO2濃度維持在4%以下；與對照組(單純打入空氣)比較，PPBRS組內的擬球藻細胞濃度提高了2.64倍；總生物質提高了2.17倍、總脂質提高了2.92倍、EPA提高了3.08倍；而期間的氧氣收集效率約為82.7%。綜合以上所述，本研究所開發的全氟碳化物光生物反應器系統組(PPBRS)提供了一個有效分離CO2、收集O2並能同時提升擬球藻生長與其經濟作物(生物質, 總脂質, EPA)產量之技術方法。;The method to reduce carbon emission and remove excessive CO2 in the atmosphere has become an international environmental issue since greenhouse effect has gained increasing attention in the world. In order to effectively remove CO2 from the mixture and simultaneously increase the economic output, a synthetic photobioreactor system, named perfluorinated photobioreactor system (PPBRS) was established in this study. The system is aimed to utilize the excellent CO2 absorption capacity of the PFC to isolate the CO2 from the mixed gas and deliver the absorbed CO2 under an appropriate ratio (v/v) to the photobioreactor for an improved microalgal growth. Furthermore, the O2 generated from the microalgae photosynthesis will be transferred through PFC adsorption and collected in another container. Our data showed that the 2% (v/v) CO2 brought by PFC may provide the highest growth rate of N. oculata compared to the ones with other settings (1 - 12% (v/v) CO2). In comparison to the cells growing with air, the cells with 2% (v/v) CO2 supply for 10 days exhibited 2-, 1.25-, 1.57-, and 1.37-fold increases of cell concentration, amounts of biomass, total lipid, and eicosapentaenoic acid (EPA), respectively. In terms of PPBRS operation for 10 days where a mixture gas of 60% N2 - 40% CO2 was employed as the model mixed gas, the results showed that the PPBRS was able to effectively isolate CO2 through PFC adsorption and maintain the CO2 concentration in the output N2 in  4% for up to 10 days. In comparison to the group with air injection throughout the time course, our data showed that the cells cultured with PPBRS provided 2.64-fold increase of cell density; 2.17-fold increase of total biomass amount; 2.92-fold increase of total lipid amount, and 3.08-fold increase of EPA production. In additional, the oxygen collection rate was about 82.7% based on the numerical evaluation. Taken together, the developed PPBRS may serve as an effective means for simultaneous CO2 separation, O2 collection, and enhanced microalgae/N. oculata productions that is highly applicable for use in the industry.
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