| 摘要: | 生醫材料與醫療器材的界面性質決定其效能、生物相容性、使用安全性與壽命， 舉例來說，因醫療器材造成的院内感染，一直是棘手的臨床問題，除了頻繁消毒與大 量的抗生素使用外，無其他有效對抗方式，卻也造成抗藥性細菌的滋生。其他如血栓、 異物反應、奈米材料上的蛋白質冠狀結構...等問題，都與材料界面性質息息相關，而 現有的表面修飾方法往往程序複雜、穩定性不佳、花費高昂與缺乏多重功能。本計劃 將建立創新生物啟發之“鄰苯二酚表面化學“(catecholic surface chemistries)， 設計具生物惰性之兩性雙離子基團與生物識別性基團的鄰笨二酚表面自組裝修飾材 料，可做快速且有效的表面改質，控制界面生物活性與吸附行為。本研究源自多巴胺 (dopamine)的多重表面吸附的特性，利用分子上的官能基氨基，轉變成其他生物功能 性基團，建構新型表面自組裝材料。初步的研究發現，經由反應溶液的酸鹼值轉換方 法（pH transition approach)，可以將雙離子型多巴胺（zwitterionic dopamine)穩 定且高密度地接枝于表面，有效抵抗蛋白質、細胞與細菌的貼附。本計劃將從材料合 成、材料物理化學學理的建立到抗菌表面與生醫檢測應用的開發，完整建構“鄰苯二 酚表面化學”之樣貌，以啟發未來更多的應用與發展。 表面自組裝材料一直對界面科學有重要影響，本研究將著力于生物啟發“鄰苯二 酚表面化學”，多面向的研究將提升界面化學與生醫材料的進步與發展。最後，本計 晝將廣泛地影響醫療產業核心技術的建立與高階人才的培養。 ;Physico-chemical properties of biointerfaces considerably determine the efficacy, biocompatibility, safety and durability of medical devices. For example, biomaterials-associated infections have been a major problem in modern medicine. Nowadays, there is no effective approach to defeat the infections, except for sterilization and uses of a plenty of antibiotics, which however contributes to the development of antibiotic resistant bacteria. The other surface-associated problems, such as thrombosis, foreign-body reaction, formation of protein corona on nanomaterials, and so on, remain troublesome. The state-of-the-art technologies for surface modification exhibit cumbersome procedure, instability, high-cost and lack of versatility. In this proposal, we aim to develop innovative bio-inspired “catecholic surface chemistries”. We will conjugate bio-inert moieties, i.e. zwitterionic groups, and bio-active moieties, i.e. biotin or folic acid, onto catecholic molecules to afford self-assembling bio-functional surface modifiers. Catecholic assemblies will effective form thin films to control interfacial properties and adsorption behaviors. The study is inspired from dopamine, which has been reported to substrate-independently adhere on surfaces. Its amine group will be converted to a variety of bio-functional moieties to construct a set of assemblies for surface engineering. Our preliminary results indicated that the zwitterionic dopamine enables to form thin films via a pH transition approach to resist non-specific adsorption of proteins, cells and bacteria. The fields of the research will cover from materials synthesis, physico-chemical fundamentals of catecholic attachment mechanism, and antimicrobial and biosensing applications. The intact contour of “catecholic surface chemistries” will be developed for their great scientific potentials and a wide spectrum of applications. Self-assembling materials have been greatly influential to surface science. The proposal will be devoted to bio-inspired “catecholic surface chemistries”. Studies from different angles will boost the advances in surface and biomaterials sciences. Last but not least, the outputs of the research will generally affect the establishment of biomedical industries and development of high-level talents. |
