開發具有抗菌、消炎、供氧及促使細胞生長特性可注射溫感性水凝膠用於慢性傷口癒合之研究;Development of Injectable Thermosensitive Hydrogel with Antibacterial, Anti-inflammatory, Oxygen-supply and Enhanced Cell Growth Properties for Chronic Wound Healing

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题名:	開發具有抗菌、消炎、供氧及促使細胞生長特性可注射溫感性水凝膠用於慢性傷口癒合之研究;Development of Injectable Thermosensitive Hydrogel with Antibacterial, Anti-inflammatory, Oxygen-supply and Enhanced Cell Growth Properties for Chronic Wound Healing
作者:	張簡博穎;Bo-Ying, Chang Chien
贡献者:	生物醫學工程研究所
关键词:	可注射水凝膠;慢性傷口癒合;抗菌;細胞生長;IL-8發炎因子;全氟辛基溴;Injectable hydrogel;Chronic wound healing;Antibacterial;Cell growth;IL-8 inflammatory factors;Perfluorooctyl bromid
日期:	2021-10-27
上传时间:	2021-12-07 11:25:31 (UTC+8)
出版者:	國立中央大學
摘要:	糖尿病是一種葡萄糖代謝異常引起的慢性疾病，它的主要特徵是血液中葡萄糖濃度的升高。而患有糖尿病的患者，其傷口稱為慢性傷口，此傷口擁有較難癒合且易受細菌感染的特性。它不僅要討論傷口中是否存在細菌感染的風險，還必須探討是否擁有促進傷口癒合的能力。在這裡，我們開發了由玻尿酸（HA）結合PEO-PPO-PEO三嵌段共聚物組成的可注射溫感性水凝膠，可改善體外慢性傷口的癒合。將人類表皮生長因子（EGF）奈米粒子、聚六亞甲基雙胍（PHMB）和全氟辛基溴化物（PFOB）奈米乳劑整合到我們的可注射溫感性水凝膠中。通過DLS分析，負載EGF的奈米顆粒和PFOB的奈米乳劑的尺寸約為973.5 ± 63.6 nm和106.16 ± 0.13 nm。為了證明其抗菌效果，選擇了人類皮膚上常見的細菌─金黃色葡萄球菌作為實驗細菌。結果表明，我們的水凝膠具有良好的抗菌能力，同時具有較高的細胞存活率和使細胞生長的能力。透過測量IL-8發炎因子，發現我們的水凝膠具有減緩發炎的能力。而在檢測氧氣的試驗中，也發現PFOB奈米乳劑確實具有攜帶氧氣的能力。總之，在這項研究中開發的可注射溫感性水凝膠在慢性傷口癒合中具有很高的潛力。;Diabetes is a chronic disease caused by abnormal glucose metabolism. Its main feature is the increase in blood glucose concentration. The wounds of diabetic patients are called chronic wounds, which are difficult to heal and are susceptible to bacterial infections. It must not only discuss whether there is a risk of bacterial infection in the wound, but also the ability to promote wound healing. Here, we developed an injectable thermosensitive hydrogel composed of PEO-PPO-PEO block co-polymer bonded with hyaluronic acid (HA) to improve the chronic wound healing. Incorporating human epidermal growth factor (EGF) nanoparticles, polyhexamethylene biguanide (PHMB) and perfluorooctyl bromide (PFOB) nano-emulsions into our injectable thermosensitive hydrogel. Through the DLS analysis, the sizes of EGF-loaded nanoparticles and PFOB nano-emulsions are about 973.5 ± 63.6 nm and 106.16 ± 0.13 nm, respectively. To demonstrate the antibacterial effect, Staphylococcus aureus, which is common focal microbiome on human skin, was selected as the experimental microorganisms. The results showed that our hydrogels had a good antibacterial ability while it gave a high cell survival rate and enhanced cell growth. By measuring IL-8 inflammatory factors, we found that our hydrogel has the ability to slow down inflammation. In the oxygen test, it was also found that PFOB nano-emulsions actually have the ability to carry oxygen. In summary, the injectable thermosensitive hydrogel developed in this study is highly potential for use in the chronic wound healing.
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