研究在擁擠環境中由多個驅動蛋白拖曳的貨物速度;Study of cargo velocity hauled by multiple kinesins in a crowded environment

Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/88265

Title:	研究在擁擠環境中由多個驅動蛋白拖曳的貨物速度;Study of cargo velocity hauled by multiple kinesins in a crowded environment
Authors:	黃雅婷;Huang, Ya-Ting
Contributors:	物理學系
Keywords:	驅動蛋白;擁擠環境;貨物速度;漲落理論;負載共享模型;Kinesin;Crowded environment;Cargo velocity;Fluctuation theorem;Shared-load model
Date:	2022-06-23
Issue Date:	2022-07-13 20:54:57 (UTC+8)
Publisher:	國立中央大學
Abstract:	Kinesin是一種依賴ATP的細胞內馬達，沿著微管長距離運輸貨物。由多個驅動蛋白牽引貨物的運輸距離被發現更遠，但是對於多個驅動蛋白從0.3到3 \textmu m/s的貨物速度沒有統一的說法，因為很少有人知道它們如何在團隊合作中工作。為了解決這個問題，我們在擁擠的環境中構建珠子測定法，並使用漲落定理判定驅動蛋白的數量，以研究擁擠效應對多個驅動蛋白拖動的貨物速度的影響。單個驅動蛋白的貨物速度被觀察到被擁擠的背景減慢。隨著驅動蛋白數量的增加，貨物速度增加並達到1 \textmu m/s的單個馬達速度，特別是在更擁擠的背景下發生顯著變化。我們的研究結果表明，驅動蛋白協同行走以分擔貨物的負載，因此在擁擠的環境中比單個馬達移動得更快，但貨物速度如何超過1 \textmu m/s的問題仍未解決。;Kinesin, an ATP-dependent intracellular motor, transports cargo along the microtubule for a long distance. It was revealed that cargo hauled by multiple kinesins travels farther, but there is no unified statement about cargo velocity with multiple kinesins from 0.3 to 3 \textmu m/s because it is rarely known how they work in teamwork. To answer this, we construct the bead assay in a crowded environment and identify the number of kinesins using the fluctuation theorem to investigate the crowding effect on cargo velocity dragged by multiple kinesins. The cargo velocity of a single kinesin is observed to be slowed by the crowded background. As the number of kinesins increases, the cargo velocity increases and reaches the single motor velocity of 1 \textmu m/s, particularly dramatically changing in the more crowded background. Our findings show that kinesins walk in collaboration to share the load on cargo and, thus, move faster than a single motor does in the crowded environment, but the question of how cargo velocity might exceed 1 \textmu m/s remains unsolved.
Appears in Collections:	[Graduate Institute of Physics] Electronic Thesis & Dissertation

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