在生化與臨床醫學等項目，透過標識分子進行檢測與分析是很重要的一種方式，而可實行於多重與高通量檢測中之標識分子可有效的提高檢測分析之功能性與量能。其中，使用編碼粒子做為光譜標記之檢測方法擔任十分重要之角色。為此，本研究論文發展出具功能化編碼之表面增強拉曼光譜標籤探針並將其於生化與臨床醫學中應用。 本研究利用拉曼散射中所展現的材料光譜獨特性，結合表面增強拉曼光譜機制，研發具有功能化編碼與可定量之標籤探針。此新型光學奈米標識分子探針，是採用特定的標記分子與貴金屬奈米粒子結合，可產生強烈的特徵拉曼光譜訊號，並藉由此獨特的光譜訊號完成編碼特性，使其具有高靈敏度、多重分析能力與可定量等獨特優點與特性。 本研究已成功完成一系列功能化之表面增強拉曼光譜標籤的合成與分析，包含合成多種編碼標籤分子以及利用不同型態之奈米材料製備出光譜增強奈米粒子，並以生物分子免疫反應之測試為例，驗證了本研究所提出之方法與系統的可行性。此研究成果提供未來在多重與高通量檢測方式之選擇外，也拓展了使用編碼粒子做為光譜標記之相關應用層面，可望在生物化學分析與臨床醫學上有更好、更廣泛的應用。;Encoded particles are one of the most powerful approaches for multiplex and high-throughput detection. The sensitive and specific analysis of biomolecules from complex mixtures is essential in the field of clinical diagnostics. The presence and progression of disease generally involve a multitude of different biomolecules. Thus detecting multiple events in tandem can reduce time and cost and obtain significantly more information from a small clinical sample. In this study, we report a universal, inexpensive, high sensitivity, and encodable synthetic protocol for fabricating a series of SERS-encoded nanoparticles. As a newly emerging optical nanoprobe, SERS-encoded nanoparticles synthesize through attaching intrinsically strong Raman scattering molecules to the surface of plasmon-resonant gold nanoparticles. It can provide strongly enhanced spectroscopic signals due to the enhancement of local optical fields at metal surfaces. With these distinctive spectroscopic signals, it is of great use in biochemical detection and analysis. The advantage including high sensitivity, easily quantitative analysis, great multiplexing capacity, and so on. Until now, our study has successfully fabricated a series of encodable functional SERS-active nanoprobes, including SERS-encoded nanoparticle, nanoaggregated, nanostars. Furthermore, through the proof-of-concept with biomolecule immune testing, the encodable functional SERS-active nanoprobes can be used for multiplex biomolecule detection. We anticipate that this novel optical nanoprobes method may expand the application of encoded particles in optical encoding systems, which is promising in biochemical multiplex and high-throughput detection.
