結合台灣本土脫鹵球菌與茭白筍殼生物炭進行三氯乙烯生物降解;Combination of water bamboo husk biochar and Dehalococcoides mccartyi for trichloroethene biodegradation

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Title:	結合台灣本土脫鹵球菌與茭白筍殼生物炭進行三氯乙烯生物降解;Combination of water bamboo husk biochar and Dehalococcoides mccartyi for trichloroethene biodegradation
Authors:	蔡晴雯;Tsai, Ching-Wen
Contributors:	生命科學系
Keywords:	三氯乙烯;生物炭;生物降解;菌相分析;Trichloroethylene;Biochar;Biodegradation;Microbial community analysis
Date:	2023-07-20
Issue Date:	2024-09-19 15:20:05 (UTC+8)
Publisher:	國立中央大學
Abstract:	三氯乙烯（TCE）是一種廣泛存在的污染物，因其持久性存在和顯著的致癌危險性而聞名。生物降解已成為移除三氯乙烯的首選方法，由於其成本低且環境友善性。然而，生物降解的效果可能受到各種環境因素的影響，例如營養源源供應不足，這可能阻礙微生物的活性，導致脫氯效果下降。所以本研究目標為提高TCE的降解效率，採用了一種協同方法，結合從台灣地下水中分離得到的Dehalococcoides mccartyi (Dhc.)菌株和茭白筍殼生物炭(BC)作為輔助吸附劑。生物炭在微生物降解系統中扮演提供營養物質、縮短污染物間的距離以及加速電子傳遞的功能，但不會改變原始微生物降解途徑。本研究結果顯示，生物炭BC600 （1 g．L-1）在一天內對400 mole L-1的TCE表現出80%的去除效率，生物炭的去除效率與生物炭的用量呈現正相關。在本研究中發現，與僅有微生物(Micro)相比，添加生物炭可以顯著提高微生物的生物降解，加速移除環境中的三氯乙烯，在10天內微生物結合生物炭(Micro-BC)的協同系統完全去除了三氯乙烯，且在40天內，三氯乙烯被微生物完整代謝成終產物乙烯。為了評估生物炭對高濃度TCE（2000 mole L-1）的影響，我們證明添加生物炭可以縮短生物降解周期並提高降解效率，在第20天時效果顯著。而next generation sequence (NGS)的資料顯示，生物炭的添加可以選擇性增加或維持一些菌的生長，像Geobacter, Dehalococcoides, Peptoclostridium, Clostridium。共同處理系統具有在高污染濃度環境下使用的潛力，因為生物炭可以降低環境中污染物的毒性，提高微生物的存活率，並進一步提高污染物的去除效果。 ;Trichloroethylene (TCE) is a widely encountered pollutant known for its persistent nature and significant carcinogenic hazards. Biodegradation has gained prominence as the preferred approach due to its cost-effectiveness and environmental compatibility. Nevertheless, biodegradation can be hindered by various environmental factors, such as inadequate energy supply, which can impede the activity of microorganisms. This study aims to investigate a synergistic approach involving the use of Dehalococcoides mccartyi (Dhc.)isolated from Taiwan and water bamboo husk biochar(BC) as an assisting adsorbent for TCE removal. BC can enhance interaction with contaminants, provide nutrients, and promote electron transfer to improve TCE decomposition, although it would not change the pathway of degradation. The results show that BC600 (1 g．L－１) exhibited an 80% removal efficiency for TCE at 400 mole．L－1 within one day. The removal efficiency was significantly dose-dependent. Compared with only microorganisms (Micro), biodegradation efficiency could be profoundly promoted by the addition of biochar. The addition of BC resulted in the complete removal of TCE within 10 days. After 40 days, TCE was completely converted to ethene in coupled with Dhc. and BC group (Micro-BC).To evaluate the effects of the biochar on a high concentration of TCE (2000 mole L-1), we proved that the addition of biochar could shorten the biodegradation period and boost degradation efficiency at day 20. The next generation sequence (NGS) data revealed that biochar addition could selectively increase or maintain the growth of some bacteria such as Geobacter, Dehalococcoides, Peptoclostridium, Clostridium A Micro-BC coupled system has the potential to be used in situations with high concentrations of pollution due to biochar′s ability to lower the toxicity of pollutants in groundwater, increase the survival rate of microorganisms, and further improve removal effectiveness.
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