開發包覆喜樹鹼-靛青綠的全氟碳外泌體用於皮膚黑色素瘤光化學治療研究;Development of perfluorocarbon exosomes encapsulated with hippuricine-indocyanine green for the photochemical treatment of cutaneous melanoma

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题名:	開發包覆喜樹鹼-靛青綠的全氟碳外泌體用於皮膚黑色素瘤光化學治療研究;Development of perfluorocarbon exosomes encapsulated with hippuricine-indocyanine green for the photochemical treatment of cutaneous melanoma
作者:	黃丞佑;Huang, Cheng-You
贡献者:	生醫科學與工程學系
关键词:	黑色素瘤;喜樹鹼;靛青綠;外泌體;全氟碳化物;可追蹤轉移性癌細胞;光治療
日期:	2023-08-16
上传时间:	2024-09-19 15:20:12 (UTC+8)
出版者:	國立中央大學
摘要:	根據2020年估計，在十種常見癌症中，非黑色素瘤皮膚癌在其中排名為男性第三名，而女性第五名，這僅僅只是非黑色素瘤癌症的新病例評估，因此，皮膚癌的治療被受到重視。皮膚癌中，黑色素瘤為最危險亞型，是一種高度侵襲性和轉移性的皮膚癌，其具有相對較高的發生率和致命性，在全球範圍內的發生率持續的上升，並且對患者的生活和健康造成了嚴重的威脅。本研究將結合傳統化學治療及新興光治療，利用光治療的方式，達到減少抗癌藥物的使用量，進而降低因大量抗癌藥物而導致患者的副作用，以此想法開發出一種載有喜樹鹼化療藥物以及靛青綠光敏劑的外泌體，且具有追蹤黑色素瘤的能力。原位注射至腫瘤部位，配合全氟碳化物，結合光治療與化學治療，兩種不同的治療方式，降低化療藥物的使用量，達到減少化學治療所導致的副作用。本研究中，所有實驗都證明了exoICP具有龐大的治療潛力，不管是在體外試驗的功能上表現，或是在體內試驗的治療上的成果，都顯示了exoICP能夠針對高轉移性的癌細胞，並且在癌細胞發生轉移時，追蹤至癌細胞處，相信在未來有機會在臨床上做出良好的成果。有趣的是，外泌體的收集方式有許多種，但是本研究發現將PFOB載入外泌體中，透過簡單的離心，即可將外泌體從溶液中分離出來，相信這對於外泌體的研究是一個重大的發現，在研究外泌體的領域中發揮出良好的效果。 ;According to 2020 estimates, non-melanoma skin cancer ranks third in men and fifth in women among the ten most common cancers, and this is only the assessment of new cases of non-melanoma cancers, so the treatment of skin cancer has been emphasized. Melanoma, the most dangerous subtype of skin cancer, is a highly aggressive and metastatic skin cancer with a relatively high incidence and fatality rate, which is increasing worldwide and poses a serious threat to patients′ lives and health. This study will combine traditional chemotherapy and emerging phototherapy to reduce the amount of anticancer drugs used and reduce the side effects caused by large amounts of anticancer drugs, with the idea of developing an exosome containing a hippocampal chemotherapeutic drug and an indocyanine-green photosensitizer with the ability to track melanomas. The exosome is injected into the tumor site with perfluorocarbons, combining phototherapy and chemotherapy, two different treatment modalities, to reduce the amount of chemotherapeutic drugs used, and to reduce the side effects caused by chemotherapy. In this study, all the experiments proved that exoICP has a huge therapeutic potential. Both the functional performance in the in vitro test and the therapeutic results in the in vivo test showed that exoICP can target highly metastatic cancer cells and track the cancer cells when they metastasize, and we believe that it has the potential to make good clinical results in the future. Interestingly, there are many ways to collect exosomes, but in this study, we found that by loading PFOB into exosomes, exosomes can be separated from solution by simple centrifugation, which is believed to be a significant discovery in the field of exosome research, and it will be effective in the field of exosome research.
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