體外仿生心臟衰竭三維模型研究;Investigation of In Vitro Bionic 3D Heart Failure Model

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Title:	體外仿生心臟衰竭三維模型研究;Investigation of In Vitro Bionic 3D Heart Failure Model
Authors:	蕭廷儒;Siao, Ting-Ru
Contributors:	生醫科學與工程學系
Keywords:	心臟衰竭;三維模型研究;heart failure;3D Heart Failure Model
Date:	2024-08-14
Issue Date:	2024-10-09 15:34:29 (UTC+8)
Publisher:	國立中央大學
Abstract:	心血管疾病當前名列全球三大死因之一，其中心力衰竭造成的死亡率偏高。因此，開發準確模擬心臟衰竭的研究模型是相當重要的。在人體內的細胞行為會與其微環境動態地相互作用，因此在實驗環境中重現這種相互作用至關重要。在這項研究中，採用甲基丙烯酸酐化豬明膠構建的三維支架可透過機械循環拉伸和壓縮來複製體內微環境。機械條件為10%應變和1.5 Hz頻率。本研究調查了三維支架內機械循環負荷對AC16人類心肌細胞的影響。研究結果表明，在動態三維支架中培養的AC16細胞在第3天和第7天表現出F-肌動蛋白、TGF-β 和YAP的表達量升高，證實了3D仿生動態培養促進細胞生長，而在動態三維支架中培養的AC16細胞在第3天和第7天心臟衰竭相關疾病BNP、CRP、desmin、myosin、TNNI3的表現量也是升高，並從細胞衍生的外泌體中發現miRNA對細胞的影響。;Cardiovascular diseases presently rank among the top three leading global causes of mortality, with heart failure representing a significant contributor to this high mortality rate. Consequently, it is imperative to develop research models that accurately simulate heart failure. Cellular behavior within the human body dynamically interacts with its microenvironment, making it essential to recreate this interaction in experimental settings. In this study, a three-dimensional scaffold constructed from porcine gelatin methacryloyl was employed to replicate the in vivo microenvironment through mechanical cyclic stretching and compression. These mechanical conditions involved a 10% strain and a 1.5 Hz frequency. The research investigated the impact of mechanical cyclic loading within the three-dimensional scaffold on AC16 human cardiomyocyte cells. The findings reveal that AC16 cells cultured within dynamic three-dimensional scaffolds exhibited heightened expression of F-actin, TGF-β, and YAP at three and seven days, providing confirmation that 3D biomimetic dynamic culture fosters cellular growth. In the dynamic three-dimensional scaffold, the AC16 cells cultured showed an increase in the expression levels of BNP, CRP, desmin, myosin, and TNNI3, which are associated with heart failure, on the 3rd and 7th days. Additionally, the impact of miRNA on the cells was observed in the exosomes derived from the cells.
Appears in Collections:	[Institute of Biomedical Engineering] Electronic Thesis & Dissertation

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